| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,628,493 | T→G | 21.7% | A321A (GCT→GCG) | livJ → | leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,628,493 | 0 | T | G | 21.7% | 100.5 / 29.4 | 69 | A321A (GCT→GCG) | livJ | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (7/47); new base G (6/9); total (13/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.78e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
CCTACGCCGCGCTGCAATCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCT > minE/2628438‑2628551 | ccTACGCCGCGCTGCAATCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAAt < 1:2583798/69‑1 (MQ=255) gcgcTGCAATCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGaaa < 1:1150801/69‑1 (MQ=255) gcgcTGCAATCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGaaa < 1:2951619/69‑1 (MQ=255) gcgcTGCAATCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGaaa < 1:2817201/69‑1 (MQ=255) gcgcTGCAATCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGaaa < 1:2461315/69‑1 (MQ=255) aTCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTc < 1:2925151/69‑1 (MQ=255) aTCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTc < 1:1104446/69‑1 (MQ=255) aTCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTc < 1:2351430/69‑1 (MQ=255) aTCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTc < 1:1598505/69‑1 (MQ=255) aTCTTTGCACGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTc < 1:1508132/69‑1 (MQ=255) cTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCg < 1:2112303/69‑1 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAgat > 1:1538122/1‑59 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGc < 1:2750631/60‑1 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg > 1:943045/1‑61 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg > 1:810366/1‑61 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg > 1:625852/1‑61 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg > 1:3072970/1‑61 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:3012695/61‑1 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg > 1:217487/1‑61 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:2910943/61‑1 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:2754822/61‑1 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:2358321/61‑1 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg > 1:1399961/1‑61 (MQ=255) tttGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:269386/61‑1 (MQ=255) cAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTg < 1:2800593/66‑1 (MQ=255) aGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGAt < 1:177519/68‑1 (MQ=255) aGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGAt < 1:593907/68‑1 (MQ=255) aGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGAt < 1:3392532/68‑1 (MQ=255) aGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGAt < 1:2491802/68‑1 (MQ=255) aGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGAt < 1:1469901/68‑1 (MQ=255) ggCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATAc < 1:683476/69‑1 (MQ=255) ggCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATAc < 1:2026585/69‑1 (MQ=255) ggCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATAc < 1:2158145/69‑1 (MQ=255) gggCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGt < 1:91666/69‑1 (MQ=255) gggCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGt < 1:2695261/69‑1 (MQ=255) gggCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGt < 1:3356279/69‑1 (MQ=255) gggCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGt < 1:2187145/69‑1 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:1702533/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:1945972/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:3286455/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:3250649/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:3115631/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:3033380/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:2991134/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:266942/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:2936418/69‑2 (MQ=255) aaTCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATgg < 1:2110076/67‑1 (MQ=255) aGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:2959647/41‑1 (MQ=255) aGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:891105/41‑1 (MQ=255) aGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:2474467/41‑1 (MQ=255) aGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:2773431/41‑1 (MQ=255) aGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:2203202/41‑1 (MQ=255) aGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCg < 1:2953149/41‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:1583574/69‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:3397302/69‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:1440106/69‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:2955784/69‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:718167/69‑1 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:1344134/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:1332954/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:1188045/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:345662/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:1400855/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:868661/1‑69 (MQ=255) gaggATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTTGATACCGTAATGGGACCGCTGa < 1:3279910/64‑1 (MQ=255) gATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCt < 1:1872837/67‑1 (MQ=255) gATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCt < 1:2686121/67‑1 (MQ=255) ccGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:3012547/61‑1 (MQ=255) ccGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:597803/61‑1 (MQ=255) | CCTACGCCGCGCTGCAATCTTTGCAGGCGGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCT > minE/2628438‑2628551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |