| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,628,532 | C→G | 24.2% | T334T (ACC→ACG) | livJ → | leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,628,532 | 0 | C | G | 24.2% | 98.3 / 29.5 | 62 | T334T (ACC→ACG) | livJ | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (6/41); new base G (6/9); total (12/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.42e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01 | |||||||||||
GGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTGACT > minE/2628466‑2628599 | gggCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGt < 1:91666/69‑1 (MQ=255)gggCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGt < 1:3356279/69‑1 (MQ=255)gggCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGt < 1:2695261/69‑1 (MQ=255)gggCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGt < 1:2187145/69‑1 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:266942/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:3286455/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:3250649/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:3115631/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:3033380/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:2991134/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:2936418/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:1945972/69‑2 (MQ=255) ggCCTGAATCAGTCTGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGtg < 1:1702533/69‑2 (MQ=255) aaTCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATgg < 1:2110076/67‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:718167/69‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:3397302/69‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:1440106/69‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:1583574/69‑1 (MQ=255) cTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:2955784/69‑1 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:1400855/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:1188045/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:868661/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:345662/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:1332954/1‑69 (MQ=255) tGACGATCCGGCGGAGATCGCTAAATACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGAc > 1:1344134/1‑69 (MQ=255) gaggATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTTGATACCGTAATGGGACCGCTGa < 1:3279910/64‑1 (MQ=255) gATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCt < 1:1872837/67‑1 (MQ=255) gATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCt < 1:2686121/67‑1 (MQ=255) ccGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:3012547/61‑1 (MQ=255) ccGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:597803/61‑1 (MQ=255) aaaTCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGt < 1:1407718/40‑1 (MQ=255) aaaTCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAg > 1:3408827/1‑69 (MQ=255) tCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCg < 1:2678938/69‑1 (MQ=255) tCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCg < 1:2424219/69‑1 (MQ=255) tCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCg < 1:2295670/69‑1 (MQ=255) tCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCg < 1:2535934/69‑1 (MQ=255) aaGCGAACTCCGTGGATACCGTAATGGGACCGCTGa < 1:2264847/36‑1 (MQ=255) aGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGa > 1:669536/1‑69 (MQ=255) tCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGct < 1:3057489/46‑2 (MQ=255) tGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTa < 1:1030716/68‑1 (MQ=255) tGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTa < 1:126400/68‑1 (MQ=255) tGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTa < 1:3331807/68‑1 (MQ=255) tGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTa < 1:2258840/68‑1 (MQ=255) tGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTa < 1:498541/68‑1 (MQ=255) tGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTa < 1:808746/68‑1 (MQ=255) ggATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTa < 1:570866/67‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCTTATTTg < 1:441742/68‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:1503317/68‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:371377/68‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:2065407/68‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:1307741/68‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:3374488/68‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:1270036/68‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:2357758/68‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:2921748/68‑1 (MQ=255) tACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:243222/68‑1 (MQ=255) aCCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:2657204/67‑1 (MQ=255) aCCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTg < 1:1969262/67‑1 (MQ=255) aCCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTGAc > 1:2072883/1‑69 (MQ=255) aCCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTGAc > 1:1435206/1‑69 (MQ=255) aCCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTGAc > 1:2656872/1‑69 (MQ=255) aCCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTGAc > 1:2510062/1‑69 (MQ=255) ccGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTGACt > 1:3111246/1‑69 (MQ=255) | GGGCCTGAATCAGTCTGACGATCCGGCTGAAATCGCCAAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGCGATCTGAAAGGCTTTGAGTTCGGCGTATTTGACT > minE/2628466‑2628599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |