| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,628,858 | C→T | 53.6% | G116G (GGG→GGA) | yhhK ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,628,858 | 0 | C | T | 53.6% | ‑2.1 / 20.4 | 28 | G116G (GGG→GGA) | yhhK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (5/8); new base T (7/8); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAATATCACTTTTGAAATCAAACTTGACGATTAACACTTCTCCCAGCCG‑CCCTGTTGTGCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATCCGCCATCCACCA > minE/2628791‑2628923 | gCAATATCACTTTTGAAATCAAACTTGACGATTAACACTTCTCCCAGCCG‑CCCTGTTGTGCCGTAAAc < 1:1147271/68‑1 (MQ=255) tatCACTTTTGAAATCAAACTTGACGATTAACACTTCTCCCAGCCG‑CCCTGTTGTGCCGTAAAcccc < 1:3377159/67‑1 (MQ=255) atCACTTTTGAAATCAAACTTGACGATTAACACTTCTCCCAGCCGCCCCTGTTGTGCCGTAAACCCCAg > 1:1259282/1‑69 (MQ=255) atCACTTTTGAAATCAAACTTGACGATTAACACTTCTCCCAGCCG‑CCCTGTTGTGCCGTAAACCCCAgc > 1:780877/1‑69 (MQ=255) atCACTTTTGAAATCAAACTTGACGATTAACACTTCTCCCAGCCG‑CCCTGTTGTGCCGTAAACCCCAgc > 1:2314009/1‑69 (MQ=255) tCACTTTTGAAATCAAACTTGACGATTAACACTTCTCCCAGCCG‑CCCTGTTGTGCCGTAAACCCCAgcg > 1:2072088/1‑69 (MQ=255) tCACTTTTGAAATCAAACTTGACGATTAACACTTCTCCCAGCCG‑CCCTGTTGTGCCGTAAACCCCAgc > 1:1426303/1‑68 (MQ=255) ttGACGATTAATGCTTCTCCCAGCCA‑CCCTGTTGTGCCGTAAATCCCAGTGCTCGCATAAACGCTGtc > 1:1161108/1‑68 (MQ=255) ttGACGATTAATGCTTCTCCCAGCCA‑CCCTGTTGTGCCGTAAATCCCAGTGCTCGCATAAACGCTGtc > 1:2864037/1‑68 (MQ=255) ttGACGATTAATGCTTCTCCCAGCCA‑CCCTGTTGTGCCGTAAATCCCAGTGCTCGCATAAACGCTGtc > 1:2475894/1‑68 (MQ=255) ttGACGATTAATGCTTCTCCCAGCCA‑CCCTGTTGTGCCGTAAATCCCAGTGCTCGCATAAACGCTGtc > 1:2377731/1‑68 (MQ=255) ttGACGATTAATGCTTCTCCCAGCCA‑CCCTGTTGTGCCGTAAATCCCAGTGCTCGCATAAACGCTGtc > 1:2279889/1‑68 (MQ=255) ttGACGATTAATGCTTCTCCCAGCCA‑CCCTGTTGTGCCGTAAATCCCAGTGCTCGCATAAACGCTGtc > 1:803898/1‑68 (MQ=255) ttGACGATTAATGCTTCTCCCAGCCA‑CCCTGTTGTGCCGTAAATCCCAGTGCTCGCATAAACGCTGtc > 1:958429/1‑68 (MQ=255) tgcTTCTCCCAGCCA‑CCCTGTTGTGCCGTAAATCCCAGTGCTCGCATAAACg < 1:632249/50‑1 (MQ=255) gtgCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATcctc < 1:1523523/67‑3 (MQ=255) gtgCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATCCGc < 1:870670/67‑1 (MQ=255) gtgCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATCCGc < 1:898170/67‑1 (MQ=255) gtgCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATCCGc < 1:990470/67‑1 (MQ=255) gtgCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATCCGc < 1:3056215/67‑1 (MQ=255) gtgCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATCCGc < 1:2993171/67‑1 (MQ=255) gtgCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATCCGc < 1:1685311/67‑1 (MQ=255) gtgCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATCCGc < 1:1422515/67‑1 (MQ=255) aaaTCCCAGTGCTCGCATAAACGCTGTCATCACGCCGCGATCTTCCACGCCAGCATCCGCCATccacca < 1:3254474/69‑1 (MQ=255) aaaTCCCAGTGCTCGCATAAACGCTGTCATCACGCCGCGATCTTCCACGCCAGCATCCGCCATccacca < 1:2727212/69‑1 (MQ=255) aaaTCCCAGTGCTCGCATAAACGCTGTCATCACGCCGCGATCTTCCACGCCAGCATCCGCCATccacca < 1:2690202/69‑1 (MQ=255) aaaTCCCAGTGCTCGCATAAACGCTGTCATCACGCCGCGATCTTCCACGCCAGCATCCGCCATccacca < 1:1895666/69‑1 (MQ=255) aaaTCCCAGTGCTAGCATAAACGCTGTCATCACGCCGCGATCTTCCACGCCAGCATCCGCCATccacca < 1:305723/69‑1 (MQ=255) aaaTCCCAGTGCTAGCATAAACGCTGTCATCACGCCGCGATCTTCCACGCCAGCATCCGCCATccacca < 1:1157856/69‑1 (MQ=255) aaaTCCCAGTGCTAGCATAAACGCTGTCATCACGCCGCGATCTTCCACGCCAGCATCCGCCATccacca < 1:1030591/69‑1 (MQ=255) | GCAATATCACTTTTGAAATCAAACTTGACGATTAACACTTCTCCCAGCCG‑CCCTGTTGTGCCGTAAACCCCAGCGCCTGCATAAACGCCGTCATCACACCGCGATCTTCCACGCCTGCATCCGCCATCCACCA > minE/2628791‑2628923 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |