Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,630,947 A→C 25.5% S54S (TCA→TCC livH → leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,630,9470AC25.5% 117.8 / 48.5 90S54S (TCA→TCClivHleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (30/37);  new base C (15/8);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCGGCTGGCTGCTGGTAGCTGCGGGAT  >  minE/2630891‑2631014
                                                        |                                                                   
aTCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGatcatc                                                         >  1:1981980/1‑69 (MQ=255)
aTCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGatcatc                                                         >  1:2902913/1‑69 (MQ=255)
aTCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGatcatc                                                         >  1:2945297/1‑69 (MQ=255)
aTCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGatcatc                                                         >  1:3130716/1‑69 (MQ=255)
aTCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGatcatc                                                         >  1:2403471/1‑69 (MQ=255)
aTCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGatcatc                                                         >  1:1452559/1‑69 (MQ=255)
aTCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGatcatc                                                         >  1:1445210/1‑69 (MQ=255)
aTCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGatcatc                                                         >  1:1397094/1‑69 (MQ=255)
aTCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTAAGatcatc                                                         >  1:3378269/1‑69 (MQ=255)
         aTCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtg                                                >  1:3097999/1‑69 (MQ=255)
         aTCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtg                                                >  1:339037/1‑69 (MQ=255)
         aTCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtg                                                >  1:1508125/1‑69 (MQ=255)
         aTCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtg                                                >  1:734193/1‑69 (MQ=255)
         aTCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtg                                                >  1:2593643/1‑69 (MQ=255)
         aTCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtg                                                >  1:2995093/1‑69 (MQ=255)
         aTCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtg                                                >  1:981489/1‑69 (MQ=255)
         aTCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtg                                                >  1:2938531/1‑69 (MQ=255)
           cAACTTCGCCCACGGCGAGGTTTACATGATTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACtgat                                              <  1:1044161/69‑1 (MQ=255)
           cAACTTCGCCCACGGCGAGGTTTACATGATTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACtgat                                              <  1:2394591/69‑1 (MQ=255)
           cAACTTCGCCCACGGCGAGGTTTACATGATTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACtgat                                              <  1:2126883/69‑1 (MQ=255)
           cAACTTCGCCCACGGCGAGGTTTACATGATTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACtgat                                              <  1:3049012/69‑1 (MQ=255)
           cAACTTCGCCCACGGCGAGGTTTACATGATTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACtgat                                              <  1:1637790/69‑1 (MQ=255)
           cAACTTCGCCCACGGCGAGGTTTACATGATTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACtgat                                              <  1:1184540/69‑1 (MQ=255)
            aaCTTCGCCCACGGCGAGGTTTACATGATTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACtgat                                              <  1:2907083/68‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:3267197/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:992384/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:109306/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:275136/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:2662906/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:2621891/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:2617699/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:249750/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:2482393/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:2409169/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:128597/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:1927477/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:2303592/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:2151821/69‑1 (MQ=255)
              cTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGGCTCATTTATGATCATCGCCGAGCtgatgat                                           <  1:2502932/69‑1 (MQ=255)
               ttCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:2679447/68‑1 (MQ=255)
               ttCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:3202010/68‑1 (MQ=255)
               ttCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:1363191/68‑1 (MQ=255)
               ttCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:398444/68‑1 (MQ=255)
                      aCGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCtgatgat                                           <  1:1273050/61‑1 (MQ=255)
                       cGGCGAGGTTTACATGATTGGCAGCTACGGCTCCTTTATGATCATCGCCGCACtgat                                              <  1:875508/57‑1 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCt                                                 >  1:1837532/1‑53 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCt                                                 >  1:2251998/1‑53 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCt                                                 >  1:2360659/1‑53 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCt                                                 >  1:3127952/1‑53 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCt                                                 >  1:3333336/1‑53 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCt                                                 >  1:289466/1‑53 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCAtt                                 <  1:1046061/69‑1 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCAtt                                 <  1:3108210/69‑1 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCAtt                                 <  1:928406/69‑1 (MQ=255)
                        ggCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCAtt                                 <  1:3072131/69‑1 (MQ=255)
                           gaagTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:3253309/66‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGTATTGAt                              <  1:4082/69‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:1051147/69‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:98237/69‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:235270/69‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:519569/69‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:2078186/69‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:1660335/69‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:3152258/69‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:3167934/69‑1 (MQ=255)
                           gAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:3183665/69‑1 (MQ=255)
                            aGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGAt                              <  1:2021063/68‑1 (MQ=255)
                                 tataTGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCggc                        >  1:1876237/1‑69 (MQ=255)
                                 tataTGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCggc                        >  1:779191/1‑69 (MQ=255)
                                 tataTGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCggc                        >  1:1905824/1‑69 (MQ=255)
                                 tataTGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCggc                        >  1:3087002/1‑69 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTTATACCggct                       >  1:2720586/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATa                             >  1:1186972/1‑58 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:567453/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:1142270/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:2631331/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:600447/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:21452/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:1611945/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:1630013/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:3113535/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:1722104/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:286924/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTGATGATGCTGGGCATTGATACCggct                       >  1:3132894/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGATCATCGCCGCACTAATGATGCTGGGCATTGATACCggct                       >  1:1433893/1‑64 (MQ=255)
                                       aTTGGCAGCTACGTCTCCTTTATGAACATCGCCGCACTGATGATGCTGGGCATTGATACCggc                        >  1:1326340/1‑63 (MQ=255)
                                           gCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCAtt                                 <  1:690985/50‑1 (MQ=255)
                                                       cATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCGGCTGGCTGCTGGTAGCTGCGGGAt  >  1:2969840/1‑69 (MQ=255)
                                                       cATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCGGCTGGCTGCTGGTAGCTGCGGGAt  >  1:645766/1‑69 (MQ=255)
                                                       cATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCGGCTGGCTGCTGGTAGCTGCGGGAt  >  1:2077674/1‑69 (MQ=255)
                                                        |                                                                   
ATCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCTCATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCGGCTGGCTGCTGGTAGCTGCGGGAT  >  minE/2630891‑2631014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: