| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,633,585 | T→C | 59.7% | N201N (AAT→AAC) | livG → | leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,633,585 | 0 | T | C | 59.7% | 21.4 / 33.0 | 52 | N201N (AAT→AAC) | livG | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (8/13); new base C (22/9); total (30/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTTAACCCGAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGATATGAAGCTGGTGATGGGAATTTCGGACCGA > minE/2633531‑2633651 | tCTTAACCCGAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAAcaccac > 1:2710397/1‑69 (MQ=255) cTTAACCCGAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAatca > 1:2462446/1‑56 (MQ=255) cTTAACCCGAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACt > 1:1641603/1‑69 (MQ=255) cTTAACCCGAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACt > 1:1167703/1‑69 (MQ=255) cccGAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATc < 1:3233050/67‑1 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAGCTGCGTAACCATCACAACACCAATATCttgtt > 1:3113578/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACt > 1:2558345/1‑61 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:3153607/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:921681/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2567161/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2580874/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2272595/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2684962/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:1148069/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2188994/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2804912/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2171162/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2153632/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2822281/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:1905807/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:3231047/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2909637/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:1507594/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:2972294/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:1449848/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgtt > 1:1398398/1‑69 (MQ=255) gAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGTAACCATCACAACACCACTATCttgat > 1:2481566/1‑67 (MQ=255) aaaGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCttgttg < 1:2321837/69‑1 (MQ=255) aaaGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCttgttg < 1:1957926/69‑1 (MQ=255) aaaGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCttgttg < 1:37593/69‑1 (MQ=255) agagCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGa < 1:792878/69‑1 (MQ=255) agagCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGa < 1:2996742/69‑1 (MQ=255) agagCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGa < 1:3069994/69‑1 (MQ=255) agagCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGa < 1:3181177/69‑1 (MQ=255) gagCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGa < 1:226850/68‑1 (MQ=255) cTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGa < 1:1163530/65‑1 (MQ=255) aGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGa > 1:1498751/1‑58 (MQ=255) cTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTTATTGAACACGATATGAAGCTGGt < 1:2786019/68‑1 (MQ=255) gATTGCCGAACTGCGTAACCATCACAACACCACTATCttg < 1:1071323/40‑1 (MQ=38) gATTGCCGAACTGCGTAACCATCACAACACCACTATCttg < 1:1034024/40‑1 (MQ=38) gATTGCCGAACTGCGTAACCATCACAACACCACTATCttg < 1:841884/40‑1 (MQ=38) gATTGCCGAACTGCGTAACCATCACAACACCACTATCttg < 1:3305591/40‑1 (MQ=38) gATTGCCGAACTGCGTAACCATCACAACACCACTATCttg < 1:3285861/40‑1 (MQ=38) gATTGCCGAACTGCGTAACCATCACAACACCACTATCttg < 1:313241/40‑1 (MQ=38) gATTGCCGAACTGCGTAACCATCACAACACCACTATCttg < 1:3025148/40‑1 (MQ=38) gATTGCCGAACTGCGTAACCATCACAACACCACTATCttg < 1:1595995/40‑1 (MQ=38) gATTGCCGAACTGCGTAACCATCACAACACCACTATCttg < 1:2182722/40‑1 (MQ=38) tGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGaa > 1:1199760/1‑46 (MQ=255) tGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGATATGAAGCTGGTGATggg > 1:449374/1‑69 (MQ=255) tGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGATATGAAGCTGGTGATggg > 1:2267485/1‑69 (MQ=255) tGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGATATGAAGCTGGTGATggg > 1:2284006/1‑69 (MQ=255) aaCTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGATATGAAGCTGGTGATGGGAAttt < 1:1413152/69‑1 (MQ=255) aaTCATCACAACACCACTATCTTGTTGATTGAACACGATATGAAGCTGGTGATGGGAATTTCGGACCGa < 1:1102617/69‑1 (MQ=255) | TCTTAACCCGAAAGAGACGAAAGAGCTGGATGAGCTGATTGCCGAACTGCGCAATCATCACAACACCACTATCTTGTTGATTGAACACGATATGAAGCTGGTGATGGGAATTTCGGACCGA > minE/2633531‑2633651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |