| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,634,911 | G→T | 31.4% | A16A (GCG→GCT) | ugpB → | glycerol‑3‑phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,634,911 | 0 | G | T | 31.4% | 64.7 / 30.4 | 54 | A16A (GCG→GCT) | ugpB | glycerol‑3‑phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (16/21); new base T (11/6); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAACAAGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTCTATGGAAGGGGAAC > minE/2634845‑2634972 | acaacaAGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGtt < 1:349104/69‑1 (MQ=255)acaacaAGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGtt < 1:2964495/69‑1 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:3038010/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa < 1:2042462/69‑1 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa < 1:2054401/69‑1 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:2057340/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:2130741/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa < 1:218670/69‑1 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa < 1:2560513/69‑1 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:2890521/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:1109508/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:3141206/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:478535/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:531355/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:533871/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:691408/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:982335/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa < 1:99874/69‑1 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa > 1:2019872/1‑69 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa < 1:135429/69‑1 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa < 1:2019942/69‑1 (MQ=255) caacaaGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTa < 1:162796/69‑1 (MQ=255) aaCGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTAATGGGGAATGcaca > 1:3316586/1‑69 (MQ=255) aaCGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTAATGGGGAATGcaca > 1:945423/1‑69 (MQ=255) aaCGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTAATGGGGAATGcaca > 1:3386718/1‑69 (MQ=255) aaCGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTAATGGGGAATGcaca > 1:1390509/1‑69 (MQ=255) caaTGAAACCGTTACGTTATACAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGcgca > 1:62210/3‑67 (MQ=255) caaTGAAACCGTTACGTTATACAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGcgca > 1:36396/3‑67 (MQ=255) gTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTgacgac < 1:1941179/69‑1 (MQ=255) tACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCa < 1:1149695/69‑1 (MQ=255) tataCAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTcc > 1:3008322/1‑68 (MQ=255) tataCAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTcc > 1:1229075/1‑68 (MQ=255) tataCAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCg > 1:1619304/1‑69 (MQ=255) tataCAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCg > 1:706968/1‑69 (MQ=255) tataCAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCg > 1:1227032/1‑69 (MQ=255) tataCAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCg > 1:2176066/1‑69 (MQ=255) tataCAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCg > 1:3153349/1‑69 (MQ=255) tataCAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCg > 1:1661524/1‑69 (MQ=255) tataCAGCTTCAGCACTGGCGCTCGGACTGGCTTTAATGGCAAATGCGCAAGCAGTGACGACCATTCCg > 1:1303927/1‑69 (MQ=255) gCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTc < 1:1047574/68‑1 (MQ=255) gCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTc < 1:636555/68‑1 (MQ=255) gCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTc < 1:593123/68‑1 (MQ=255) gCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTc < 1:135184/68‑1 (MQ=255) gCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTc < 1:3365996/68‑1 (MQ=255) gCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTc < 1:1508770/68‑1 (MQ=255) gCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTc < 1:1531690/68‑1 (MQ=255) gCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTc < 1:2772621/68‑1 (MQ=255) gCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTCACGACCATTCCGTTCTGGCATTc < 1:2989662/68‑1 (MQ=255) aCTGGCTTTAATGGCAATTGCGCAGGCAGTGACGACCATTCCGTTCTGGCATTCTATGGAAGGGGAAc < 1:1545621/68‑1 (MQ=255) aCTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCGTTCTGGCATTCTATGGAAGGGGAAc < 1:514084/68‑1 (MQ=255) aCTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCGTTCTGGCATTCTATGGAAGGGGAAc < 1:20607/68‑1 (MQ=255) aCTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCGTTCTGGCATTCTATGGAAGGGGAAc < 1:2962540/68‑1 (MQ=255) aCTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCGTTCTGGCATTCTATGGAAGGGGAAc < 1:1954225/68‑1 (MQ=255) aCTGGCTTTAATGGCAAATGCGCAGGCAGTGACGACCATTCCGTTCTGGCATTCTATGGAAGGGGAAc < 1:965446/68‑1 (MQ=255) | ACAACAAGAGAGATAAACGATGAAACCGTTACATTATACAGCTTCAGCACTGGCGCTCGGACTGGCGTTAATGGGGAATGCACAGGCAGTGACGACCATTCCGTTCTGGCATTCTATGGAAGGGGAAC > minE/2634845‑2634972 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |