| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,635,184 | G→A | 44.4% | V107V (GTG→GTA) | ugpB → | glycerol‑3‑phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,635,184 | 0 | G | A | 44.4% | 25.2 / 77.4 | 99 | V107V (GTG→GTA) | ugpB | glycerol‑3‑phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (47/8); new base A (27/17); total (74/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.75e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GGTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTTACTACTC > minE/2635121‑2635249 | ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:104145/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:2151874/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:2136162/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:2099145/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:2739162/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:2029618/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:2771076/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:3123791/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:3307282/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:3370298/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:519834/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:1551207/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:1431797/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:720270/1‑66 (MQ=255)ggTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTAtt > 1:823394/1‑66 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:1689890/1‑69 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:1043289/1‑69 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:851261/1‑69 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:1874246/1‑69 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:3038099/1‑69 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:3124682/1‑69 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:164440/1‑69 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:2195986/1‑69 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:1271684/1‑69 (MQ=255) ttATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAaga > 1:550158/1‑69 (MQ=255) ttGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGa > 1:797050/1‑69 (MQ=255) gacaccaTGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:2526991/3‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc < 1:2303653/69‑1 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:2485409/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:2497750/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:561479/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:937175/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:2677607/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:907316/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:2767083/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:763614/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:751544/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:661506/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:1862444/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:2036731/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc < 1:1623346/69‑1 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:139946/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:1382443/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:122910/1‑69 (MQ=255) gCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTc > 1:2234223/1‑69 (MQ=255) atgGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGt > 1:508321/1‑67 (MQ=255) atgGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGt > 1:2421911/1‑67 (MQ=255) atgGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGt > 1:2425892/1‑67 (MQ=255) atgGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGt > 1:647711/1‑67 (MQ=255) atgGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGt > 1:1004753/1‑67 (MQ=255) atgGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGt > 1:1462410/1‑67 (MQ=255) ggCGTCGAAAGCCATTAAACCGTTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:2159109/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:919307/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:3077734/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:309979/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:3133454/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:1772519/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:2600202/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:3313988/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:2351371/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:1118328/69‑1 (MQ=255) ggCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:333016/69‑1 (MQ=255) ggCGTCCAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:2476355/69‑1 (MQ=255) gCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:983880/68‑1 (MQ=255) gCGTCGAAAGCCATTAAACCGGTGTATGACGTATTTAAAGAGGCAGGGATTCAATTCGATGAGTCGCa < 1:2214973/68‑1 (MQ=255) cATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCg > 1:2746105/1‑67 (MQ=255) cATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGAc > 1:1147054/1‑69 (MQ=255) cATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGAc > 1:486633/1‑69 (MQ=255) cATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGAc < 1:1647453/69‑1 (MQ=255) cATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGAc < 1:426181/69‑1 (MQ=255) cATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGAc < 1:3428570/69‑1 (MQ=255) cATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGAc < 1:2062684/69‑1 (MQ=255) cATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGAc < 1:856060/69‑1 (MQ=255) tAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGt < 1:1317922/69‑1 (MQ=255) aaaCCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGt > 1:1168138/1‑68 (MQ=255) aaaCCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGt > 1:3381885/1‑68 (MQ=255) aaaCCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGt > 1:3170744/1‑68 (MQ=255) aaaCCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGt > 1:2842127/1‑68 (MQ=255) aaaCCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGt > 1:1109374/1‑68 (MQ=255) tGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCg > 1:2311581/1‑52 (MQ=255) tGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCg > 1:2510052/1‑52 (MQ=255) tGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTtacta > 1:917856/1‑69 (MQ=255) tGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTtacta > 1:2344087/1‑69 (MQ=255) tGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTtacta > 1:704481/1‑69 (MQ=255) tGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTtacta > 1:2494965/1‑69 (MQ=255) tGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTtacta > 1:556582/1‑69 (MQ=255) tGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTtacta > 1:493824/1‑69 (MQ=255) tGAAGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTtacta > 1:1742981/1‑69 (MQ=255) gtgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTTACTACTc > 1:2552232/1‑68 (MQ=255) tgtTTAAAGAGGCAGTGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:2364938/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:1150676/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:3177983/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:1370138/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:1590306/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:1595643/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:3318518/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:223557/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:2823938/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:1993187/59‑1 (MQ=255) tgtTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGt < 1:2242220/59‑1 (MQ=255) | GGTTTATGAAGTTGGCACCGCCACCATGATGGCGTCGAAAGCCATTAAACCGGTGTATGACGTGTTTAAAGAGGCAGGGATTCAGTTCGATGAGTCGCAGTTTGTGCCGACGGTTTCAGGTTACTACTC > minE/2635121‑2635249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |