| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,635,532 | G→T | 61.3% | L223L (CTG→CTT) | ugpB → | glycerol‑3‑phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,635,532 | 0 | G | T | 61.3% | 16.9 / 38.9 | 44 | L223L (CTG→CTT) | ugpB | glycerol‑3‑phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/15); new base T (13/14); total (15/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GCCTGGAACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAGG > minE/2635470‑2635593 | gcCTGGAACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTc < 1:2337356/69‑1 (MQ=255)gcCTGGAACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTc < 1:742324/69‑1 (MQ=255)gcCTGGAACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTc < 1:1154577/69‑1 (MQ=255)gcCTGGAACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTc < 1:633590/69‑1 (MQ=255)gcCTGGAACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTc < 1:190903/69‑1 (MQ=255)gcCTGGAACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTc < 1:3341994/69‑1 (MQ=255)gcCTGGAACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTc < 1:3084270/69‑1 (MQ=255) aCGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGc < 1:1462678/69‑1 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGa < 1:1933096/69‑1 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGa < 1:2486446/69‑1 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:312943/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:26628/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:2676853/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:2996154/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1020079/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:337109/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:979940/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:980859/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1016145/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1961976/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:115283/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1467981/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1530980/1‑69 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:1055047/69‑1 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:1773103/69‑1 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:1562176/69‑1 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:2772623/69‑1 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:1676960/69‑1 (MQ=255) aaaCAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGc < 1:2999672/69‑1 (MQ=255) aaaCAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGc < 1:2907028/69‑1 (MQ=255) aaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCTGAGCAGGTGAAACACATCg < 1:3321058/68‑1 (MQ=255) aaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:2480595/68‑1 (MQ=255) aacaacGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGcc < 1:2775028/69‑1 (MQ=255) aacaacGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGcc < 1:1466742/69‑1 (MQ=255) aCGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCgagga < 1:2272833/67‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:1964569/68‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:1633244/68‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:2768299/68‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:1734601/68‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:401411/68‑1 (MQ=255) gACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:1734034/68‑1 (MQ=255) gACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:2260697/68‑1 (MQ=255) cgGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACaagaag > 1:322717/1‑68 (MQ=255) cgGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAgg > 1:733947/1‑69 (MQ=255) | GCCTGGAACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAGG > minE/2635470‑2635593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |