| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,635,541 | T→C | 64.4% | N226N (AAT→AAC) | ugpB → | glycerol‑3‑phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,635,541 | 0 | T | C | 64.4% | 26.4 / 21.2 | 42 | N226N (AAT→AAC) | ugpB | glycerol‑3‑phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (5/10); new base C (13/14); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAGGGCGACTTCAGCTA > minE/2635477‑2635606 | aCGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGc < 1:1462678/69‑1 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGa < 1:2486446/69‑1 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGa < 1:1933096/69‑1 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:980859/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1016145/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:979940/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:337109/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:312943/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:2996154/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:2676853/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:26628/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1961976/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1020079/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:115283/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1467981/1‑69 (MQ=255) ttGCCAGCAAAAACAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGa > 1:1530980/1‑69 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:1773103/69‑1 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:1055047/69‑1 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:2772623/69‑1 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:1562176/69‑1 (MQ=255) aaaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:1676960/69‑1 (MQ=255) aaaCAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGc < 1:2907028/69‑1 (MQ=255) aaaCAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGc < 1:2999672/69‑1 (MQ=255) aaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCTGAGCAGGTGAAACACATCg < 1:3321058/68‑1 (MQ=255) aaaCAACGGCTTTGACGGCACAGACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCg < 1:2480595/68‑1 (MQ=255) aacaacGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGcc < 1:1466742/69‑1 (MQ=255) aacaacGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGcc < 1:2775028/69‑1 (MQ=255) aCGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCgagga < 1:2272833/67‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:1734601/68‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:401411/68‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:1964569/68‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:2768299/68‑1 (MQ=255) agACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:1633244/68‑1 (MQ=255) gACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:2260697/68‑1 (MQ=255) gACGCGGTGCTTGAGTTCAACAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAAGAGATGAACaa < 1:1734034/68‑1 (MQ=255) cgGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACaagaag > 1:322717/1‑68 (MQ=255) cgGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAgg > 1:733947/1‑69 (MQ=255) gTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAGGGCGACTTCAg < 1:1970751/69‑1 (MQ=255) ttCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAGGGCGACTTCAg < 1:3193690/68‑1 (MQ=255) cAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACaaga > 1:2926947/1‑53 (MQ=255) cAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAGGGCGACTTCAGCTa > 1:3019830/1‑69 (MQ=255) cAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAGGGCGACTTCAGCTa > 1:2761491/1‑69 (MQ=255) | ACGGTCTGCCGTTTGCCAGCAAAAACAACGGCTTTGACGGCACGGACGCGGTGCTGGAGTTCAATAAGCCGGAGCAGGTGAAACACATCGCCATGCTCGAGGAGATGAACAAGAAGGGCGACTTCAGCTA > minE/2635477‑2635606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |