Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,636,862 C→T 73.7% D195D (GAC→GAT ugpA → glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,636,8620CT73.7% 55.3 / 18.5 53D195D (GAC→GATugpAglycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (6/8);  new base T (7/32);  total (13/40)
Fisher's exact test for biased strand distribution p-value = 8.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

ACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTTTCTTC  >  minE/2636795‑2636928
                                                                   |                                                                  
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:1040494/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:855334/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:3353191/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:3306490/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:2797918/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:120778/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:2644620/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:2570204/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:137021/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:1377042/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:2434634/68‑1 (MQ=255)
aCTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:1387030/68‑1 (MQ=255)
 cTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGACgg                                                                  <  1:987735/69‑1 (MQ=255)
 cTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGACgg                                                                  <  1:2316602/69‑1 (MQ=255)
          cttctATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGAc                                                                    <  1:2124938/58‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:1031471/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:2667600/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:2663534/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:300629/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:3080323/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:3366540/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:2469017/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:1383570/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:483398/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:1387840/66‑1 (MQ=255)
                  ccGCGCTGCAATCCATCCCCCGTGCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:2004674/66‑1 (MQ=255)
                   cgcgCTGCAATCCATCCCCCGTTCGTTGATCGAAGCGGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:1071791/65‑1 (MQ=255)
                   cgcgCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:1770924/65‑1 (MQ=255)
                   cgcgCTGCAATCCATCCCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:2682205/65‑1 (MQ=255)
                         gCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGACTGTGCAGGGCCGATTCGCCGcttct                                           <  1:1161939/68‑1 (MQ=255)
                            atccatccCCCGTTCGTTGATCGAAGCCGCAGCCATTGATGGGGCAGGGCAGATTc                                                    <  1:2918188/56‑1 (MQ=255)
                                                 cGAAGCCGCAGCCATTGATGGGGCAGGGCCGATTc                                                    <  1:202259/35‑1 (MQ=25)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:493147/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:545134/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:68693/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:1118285/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:698173/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:2751717/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:1552493/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:1516548/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:1966547/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:2103652/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:2327126/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:1888823/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:1262664/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:259481/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGCCGCtgc                      <  1:1238964/63‑3 (MQ=255)
                                                   aaGCCTCAGCAATGGATGGTGCAGGTCCATTCCAGAACTTCTTTAAGATTACGCTGACGCtgc                      <  1:4354/63‑3 (MQ=255)
                                                    aGCCGCAGCCATTGATGGGGCAGGGCTGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGctc                >  1:3248129/1‑66 (MQ=255)
                                                    aGCCGCAGCCATTGATGGGGCAGGGCCGATTCGGCGCTTCTTTAAGATTGCGCTGCCGCTTATCGctc                >  1:1920997/1‑66 (MQ=255)
                                                    aGCCGCAGCCATTGATGGGGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGctc                >  1:258059/1‑66 (MQ=255)
                                                    aGCCGCAGCCATTGATGGGGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGctc                >  1:3288815/1‑66 (MQ=255)
                                                    aGCCGCAGCCATTGATGGGGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGctc                >  1:248320/1‑66 (MQ=255)
                                                    aGCCGCAGCCATTGATGGGGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGctc                >  1:2470867/1‑66 (MQ=255)
                                                    aGCCGCAGCCATTGATGGGGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGctc                >  1:2410805/1‑66 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttcttc  >  1:2898814/1‑69 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttcttc  >  1:842307/1‑69 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttct    >  1:97823/1‑67 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttct    <  1:847265/67‑1 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttct    <  1:1088811/67‑1 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttct    <  1:3425775/67‑1 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttct    >  1:3258402/1‑67 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttct    >  1:2492884/1‑67 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttct    >  1:2848740/1‑67 (MQ=255)
                                                                 gACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttct    <  1:2733979/67‑1 (MQ=255)
                                                                  aCGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTttct    <  1:2693042/66‑1 (MQ=255)
                                                                   |                                                                  
ACTTCCTGTTCTTCTATGCCGCGCTGCAATCCATTCCCCGTTCGTTGATCGAAGCCGCAGCCATCGACGGTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTTTCTTC  >  minE/2636795‑2636928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: