Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,638,461 C→T 54.8% R151R (CGC→CGT ugpC → glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,638,4610CT54.8% ‑1.4 / 22.5 31R151R (CGC→CGTugpCglycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (4/10);  new base T (7/10);  total (11/20)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.27e-01

ACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCA  >  minE/2638395‑2638523
                                                                  |                                                              
aCGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGa                                                              <  1:2547837/69‑1 (MQ=255)
aCGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGa                                                              <  1:3277320/69‑1 (MQ=255)
aCGTCGCCAGCGCGAGCTTTCCGGCGGGCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGa                                                              <  1:1304209/69‑1 (MQ=255)
        cgcgcgAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCcggcgg                                                      <  1:2291219/69‑1 (MQ=255)
         gcgcgAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGAGCGCGATCcggcgg                                                      <  1:3152332/68‑1 (MQ=255)
                                  cAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGctctct                            <  1:2921008/69‑1 (MQ=255)
                                   aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGctctct                            >  1:2012827/1‑68 (MQ=255)
                                   aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa                           >  1:72712/1‑69 (MQ=255)
                                   aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa                           >  1:693940/1‑69 (MQ=255)
                                   aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa                           >  1:3314261/1‑69 (MQ=255)
                                   aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa                           >  1:2159279/1‑69 (MQ=255)
                                   aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa                           >  1:3095589/1‑69 (MQ=255)
                                   aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa                           >  1:2815308/1‑69 (MQ=255)
                                   aGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTtg                                          >  1:1424517/1‑54 (MQ=255)
                                   aGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTa                           >  1:192388/1‑69 (MQ=255)
                                   aGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTa                           >  1:2756186/1‑69 (MQ=255)
                                       tgtgGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAAcc                        >  1:2319671/1‑68 (MQ=255)
                                                          aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:952664/68‑1 (MQ=25)
                                                          aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:1299155/68‑1 (MQ=25)
                                                          aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:2980028/68‑1 (MQ=25)
                                                          aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:2861343/68‑1 (MQ=25)
                                                          aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:2770717/68‑1 (MQ=25)
                                                          aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:2381910/68‑1 (MQ=25)
                                                          aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:1993222/68‑1 (MQ=25)
                                                          aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:1656422/68‑1 (MQ=25)
                                                          aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:1636444/68‑1 (MQ=25)
                                                           ttGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt     <  1:1945034/67‑1 (MQ=25)
                                                            tgtgCGCGATCCGGCTGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCa  <  1:2621889/69‑1 (MQ=255)
                                                            tgtgCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCa  <  1:2701989/69‑1 (MQ=255)
                                                            tgtgCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCa  <  1:3274769/69‑1 (MQ=255)
                                                            tgtgCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCa  <  1:383349/69‑1 (MQ=255)
                                                                  |                                                              
ACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCA  >  minE/2638395‑2638523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: