| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,638,464 | T→G | 60.7% | D152E (GAT→GAG) | ugpC → | glycerol‑3‑phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,638,464 | 0 | T | G | 60.7% | 12.3 / 20.1 | 28 | D152E (GAT→GAG) | ugpC | glycerol‑3‑phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (4/7); new base G (7/10); total (11/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01 | |||||||||||
CGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCA > minE/2638403‑2638523 | cgcgcgAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCcggcgg < 1:2291219/69‑1 (MQ=255) gcgcgAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGAGCGCGATCcggcgg < 1:3152332/68‑1 (MQ=255) cAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGctctct < 1:2921008/69‑1 (MQ=255) aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGctctct > 1:2012827/1‑68 (MQ=255) aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa > 1:72712/1‑69 (MQ=255) aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa > 1:693940/1‑69 (MQ=255) aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa > 1:3314261/1‑69 (MQ=255) aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa > 1:3095589/1‑69 (MQ=255) aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa > 1:2159279/1‑69 (MQ=255) aGCGTGTGGCGATGGGGCGTGCGATTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTa > 1:2815308/1‑69 (MQ=255) aGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTtg > 1:1424517/1‑54 (MQ=255) aGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTa > 1:2756186/1‑69 (MQ=255) aGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTa > 1:192388/1‑69 (MQ=255) tgtgGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAAcc > 1:2319671/1‑68 (MQ=255) aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:1299155/68‑1 (MQ=25) aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:2770717/68‑1 (MQ=25) aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:2381910/68‑1 (MQ=25) aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:2861343/68‑1 (MQ=25) aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:2980028/68‑1 (MQ=25) aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:1993222/68‑1 (MQ=25) aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:1656422/68‑1 (MQ=25) aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:1636444/68‑1 (MQ=25) aTTGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:952664/68‑1 (MQ=25) ttGTGCGTGAGCCGGCAGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGTTGCgtgt < 1:1945034/67‑1 (MQ=25) tgtgCGCGATCCGGCTGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCa < 1:2621889/69‑1 (MQ=255) tgtgCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCa < 1:2701989/69‑1 (MQ=255) tgtgCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCa < 1:3274769/69‑1 (MQ=255) tgtgCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCa < 1:383349/69‑1 (MQ=255) | CGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCA > minE/2638403‑2638523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |