| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 224,636 | G→A | 20.4% | F153F (TTC→TTT) | pepD ← | aminoacyl‑histidine dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 224,636 | 0 | G | A | 20.4% | 162.4 / 27.7 | 107 | F153F (TTC→TTT) | pepD | aminoacyl‑histidine dipeptidase |
| Reads supporting (aligned to +/- strand): ref base G (59/26); new base A (13/9); total (72/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
TTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGT > minE/224569‑224703 | tttCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCg < 1:1295454/68‑1 (MQ=255)tttCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCg < 1:2912125/68‑1 (MQ=255) ccTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGc < 1:661871/68‑1 (MQ=255) cttcttcttCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGc < 1:1539024/67‑1 (MQ=255) ttcttcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGc > 1:38698/1‑66 (MQ=255) ttcttcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa > 1:1004836/1‑67 (MQ=255) ttcttcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa > 1:871337/1‑67 (MQ=255) ttcttcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa > 1:1526417/1‑67 (MQ=255) ttcttcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa > 1:1391482/1‑67 (MQ=255) ttcttcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa > 1:2294718/1‑67 (MQ=255) ttcttcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa > 1:2688715/1‑67 (MQ=255) ttcttcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa > 1:1348386/1‑67 (MQ=255) tcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc < 1:1654193/68‑1 (MQ=255) tcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc < 1:150064/68‑1 (MQ=255) tcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc < 1:2124441/68‑1 (MQ=255) tcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc < 1:2377038/68‑1 (MQ=255) tcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc < 1:3326092/68‑1 (MQ=255) tcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc < 1:1137689/68‑1 (MQ=255) tcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc < 1:739599/68‑1 (MQ=255) tcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc < 1:1094765/68‑1 (MQ=255) gTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCGTCCATACCGGc > 1:859298/1‑69 (MQ=255) gTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCGTCCATACCGGc > 1:1807969/1‑69 (MQ=255) gTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTT‑CTCTGTAAGCCAAAAGCACCGTCCATACCgg > 1:682618/1‑67 (MQ=255) ttAATCAGGATATCAGCTTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:3219628/69‑1 (MQ=255) ttAATCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:2744463/69‑1 (MQ=255) ttAATCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:3045671/69‑1 (MQ=255) ttAATCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:573628/69‑1 (MQ=255) ttAATCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:1815160/69‑1 (MQ=255) ttAATCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:938018/69‑1 (MQ=255) ttAATCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:1303504/69‑1 (MQ=255) ttAATCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:1166439/69‑1 (MQ=255) ttAATCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:1543365/69‑1 (MQ=255) ttAATCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTACg < 1:1548927/69‑1 (MQ=255) ttAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg > 1:1856362/1‑69 (MQ=255) ttAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg > 1:1270680/1‑69 (MQ=255) aaTCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCa > 1:4259/1‑53 (MQ=255) aaTCAGAATATCAGCCTGCAACGAGTTGCCCTGTAAGCCGAACGCACCGTCCa > 1:2165520/1‑53 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGACCGCACCGTCCa > 1:788314/1‑53 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCAcc > 1:1098183/1‑48 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCg > 1:908817/1‑49 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGt > 1:2358023/1‑50 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGt > 1:2024897/1‑50 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc > 1:3289568/1‑51 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc > 1:3276936/1‑51 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc > 1:2565480/1‑51 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc > 1:713385/1‑51 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc > 1:2931017/1‑51 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc > 1:1504254/1‑51 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc > 1:2061079/1‑51 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc > 1:3152705/1‑51 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc > 1:1970493/1‑51 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCa > 1:1322524/1‑53 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCa > 1:3118988/1‑53 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCa > 1:2515598/1‑53 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCa > 1:2679248/1‑53 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCa > 1:1358281/1‑53 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGt > 1:2694453/1‑69 (MQ=255) aaTCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCAGAACGCACCGTCCa > 1:1665854/1‑53 (MQ=255) tCAGGATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg < 1:1830333/65‑1 (MQ=255) aGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagcag > 1:449136/1‑69 (MQ=255) aGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagcag > 1:2642801/1‑69 (MQ=255) aGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagca > 1:1249129/1‑68 (MQ=255) ccTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCg > 1:1800126/1‑35 (MQ=25) ccTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCg > 1:2727189/1‑35 (MQ=25) ccTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCg > 1:2572212/1‑35 (MQ=25) ccTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCg > 1:3384165/1‑35 (MQ=25) ccTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCg > 1:1378884/1‑35 (MQ=25) ccTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCg > 1:1445314/1‑35 (MQ=25) ccTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCg > 1:1926898/1‑35 (MQ=25) ccTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCg > 1:10409/1‑35 (MQ=25) ccTGCAACCAGTTGCTCTGTAAGCCAAAAGCACCg > 1:88005/1‑35 (MQ=25) cTGTAAGCCAAAAGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGTACTTCCAGCGGGCCGTg < 1:2711436/69‑1 (MQ=255) cTGTAAGCCAAAAGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTg < 1:1115941/69‑1 (MQ=255) cTGTAAGCCAAAAGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTg < 1:508695/69‑1 (MQ=255) cTGTAAGCCAAAAGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTg < 1:451160/69‑1 (MQ=255) cTGTAAGCCAAAAGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTg < 1:197814/69‑1 (MQ=255) cTGTAAGCCAAAAGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTg < 1:1073706/69‑1 (MQ=255) cTGTAAGCCAAAAGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTg < 1:1360808/69‑1 (MQ=255) tGTAAGCCAAAAGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTg < 1:2986785/68‑1 (MQ=255) tGTAAGCCAAAAGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTg < 1:184149/68‑1 (MQ=255) tAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAAc > 1:1018518/1‑69 (MQ=255) tAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAAc > 1:2176721/1‑69 (MQ=255) tAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAAc > 1:124576/1‑69 (MQ=255) tAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAAc > 1:2476439/1‑69 (MQ=255) tAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAAc > 1:2815213/1‑69 (MQ=255) tAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAAc > 1:3193324/1‑69 (MQ=255) tAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAAc > 1:1317149/1‑69 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcag > 1:2701149/1‑40 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca > 1:1706019/1‑45 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca > 1:1699401/1‑45 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca > 1:2319922/1‑45 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca < 1:1098095/45‑1 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca > 1:1988907/1‑45 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca > 1:2110755/1‑45 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca > 1:2360534/1‑45 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca < 1:3343151/45‑1 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca > 1:2901023/1‑45 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAgcagca > 1:3115906/1‑45 (MQ=255) cGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGt > 1:1577625/1‑69 (MQ=255) cGAACCCACCGTCCATACCGGCTTCTTCGGTCATGGTaagc > 1:386396/1‑41 (MQ=255) | TTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGT > minE/224569‑224703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |