| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,639,637 | A→C | 68.8% | I188L (ATT→CTT) | ugpQ → | glycerophosphodiester phosphodiesterase, cytosolic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,639,637 | 0 | A | C | 68.8% | 36.5 / 16.1 | 32 | I188L (ATT→CTT) | ugpQ | glycerophosphodiester phosphodiesterase, cytosolic |
| Reads supporting (aligned to +/- strand): ref base A (5/5); new base C (8/14); total (13/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
TGTTGCTGGATGAGTGGCGCGACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCAGTTGAAA > minE/2639575‑2639687 | tgttgCTGGATGAGTGGCGCGACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTa > 1:2164859/1‑63 (MQ=255)tgttgCTGGATGAGTGGCGCGACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTa > 1:2160954/1‑63 (MQ=255)tgttgCTGGATGAGTGGCGCGACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTa > 1:1917953/1‑63 (MQ=255)tgttgCTGGATGAGTGGAGCGACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTa > 1:2561067/1‑63 (MQ=255) ggATGAGTGGCGCGACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCa < 1:3195983/69‑1 (MQ=255) gcgACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTc > 1:978080/1‑68 (MQ=255) gcgACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTc > 1:2934293/1‑68 (MQ=255) gcgACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTc > 1:2659256/1‑68 (MQ=255) gcgACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTc > 1:2325065/1‑68 (MQ=255) gcgACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTc > 1:2105707/1‑68 (MQ=255) gACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCa < 1:2587221/69‑1 (MQ=255) gACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCa < 1:334081/69‑1 (MQ=255) gACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCa < 1:1015942/69‑1 (MQ=255) gACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCa < 1:2144460/69‑1 (MQ=255) cgcgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAg > 1:1637151/1‑38 (MQ=255) cgcgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAg > 1:1003859/1‑38 (MQ=255) cgcgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAg > 1:1601029/1‑38 (MQ=255) cgcgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAg > 1:1594541/1‑38 (MQ=255) cgcgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAg > 1:3022304/1‑38 (MQ=255) cgcgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAg > 1:723217/1‑38 (MQ=255) cgcgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAg > 1:3157644/1‑38 (MQ=255) cgcgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAg > 1:3374531/1‑38 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:3139206/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:945955/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:849612/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:838186/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:621546/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:3390829/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:1387852/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:1559734/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:3181576/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:3124378/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:3122740/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:181573/69‑1 (MQ=255) cgGCTGGGCTGCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:1910331/69‑1 (MQ=255) ggctgggctgCGTCTCTCTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:1785483/68‑1 (MQ=255) tctcTTCATCTCAATCATAAGTTACTCGATAAAGCGCGAGTGATGCAGTTGaaa < 1:2284766/54‑1 (MQ=39) | TGTTGCTGGATGAGTGGCGCGACGACTGGCGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCAGTTGAAA > minE/2639575‑2639687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |