| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,641,322 | C→T | 50.7% | S319S (AGC→AGT) | ggt → | gamma‑glutamyltranspeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,641,322 | 0 | C | T | 50.7% | ‑4.5 / 62.6 | 69 | S319S (AGC→AGT) | ggt | gamma‑glutamyltranspeptidase |
| Reads supporting (aligned to +/- strand): ref base C (13/21); new base T (13/22); total (26/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
CCATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGAAATACGCCTACGCCGACCGCTCGGAATAT > minE/2641259‑2641385 | tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:1396951/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:399493/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:1904553/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:1863079/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:455998/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:2129363/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:3261019/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:127379/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:2342589/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:2520212/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:1166506/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcga > 1:1153412/2‑69 (MQ=255)tcATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGCAGAAGTACGGCTTTGGCAGTGCcg > 1:538274/2‑68 (MQ=255) aaTCCTCAATATTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAAt < 1:3169212/69‑1 (MQ=255) aaTCCTCAATATTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAAt < 1:2568139/69‑1 (MQ=255) aaTCCTCAATATTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAAt < 1:213073/69‑1 (MQ=255) aaTCCTCAATATTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAAt < 1:3288293/69‑1 (MQ=255) aaTCCTCAATATTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAAt < 1:485042/69‑1 (MQ=255) aaTCCTCAATATTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAAt < 1:1301854/69‑1 (MQ=255) aaTCCTCAATATTCCGGAAAACCTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAAt < 1:3145921/69‑1 (MQ=255) tatTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGa > 1:2816812/1‑69 (MQ=255) tatTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGa > 1:2230892/1‑69 (MQ=255) tatTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGa > 1:2234848/1‑69 (MQ=255) aaaCTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGgaga < 1:3358199/68‑1 (MQ=255) aaaCTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGgaga < 1:1297889/68‑1 (MQ=255) aaCTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa < 1:2260430/69‑1 (MQ=255) aaCTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa < 1:2829666/69‑1 (MQ=255) aaCTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa < 1:2865501/69‑1 (MQ=255) aaCTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa < 1:3284466/69‑1 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCTGAGaaa < 1:440372/67‑1 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa > 1:343878/1‑67 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa > 1:694863/1‑67 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa < 1:3108234/67‑1 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa > 1:1150910/1‑67 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa > 1:577503/1‑67 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa > 1:967298/1‑67 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa < 1:1223491/67‑1 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa > 1:1424997/1‑67 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa > 1:1538958/1‑67 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa > 1:2808215/1‑67 (MQ=255) cTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa < 1:228217/67‑1 (MQ=255) tCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGgaga < 1:2346942/63‑1 (MQ=255) atatGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGAAAtacgcc < 1:2244549/68‑1 (MQ=255) atatGAAGAAATAAGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGAAAtacgcc < 1:1937652/68‑1 (MQ=255) aagaaATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGAAAtacgcctacgcc > 1:3297849/1‑69 (MQ=255) aagaaATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGAAAtacgcctacgcc > 1:3414064/1‑69 (MQ=255) ggCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGaaa < 1:2814485/48‑1 (MQ=255) gCAGTGCCGACGCGATGCTAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2880599/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATGCGCCTATGCCGACCGCTCGGAatat < 1:2468854/68‑1 (MQ=37) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGTAatat < 1:69728/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:1581933/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:3022625/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:871514/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:737509/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:73118/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:1234178/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:675432/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2560568/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2545132/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2972004/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:1748608/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2304746/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:1957495/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2014555/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:3259687/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2024625/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2888427/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2093237/68‑1 (MQ=255) gCAGTGCCGACGCGATGCAAATCATGGCAGAAGCTGAGAAATACGCCTATGCCGACCGCTCGGAatat < 1:2181940/68‑1 (MQ=255) | CCATATCGTACAAATCCTCAATATTCTGGAAAACTTCGATATGAAGAAATACGGCTTTGGCAGCGCCGATGCGATGCAAATCATGGCAGAAGCGGAGAAATACGCCTACGCCGACCGCTCGGAATAT > minE/2641259‑2641385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |