| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,641,712 | T→C | 68.1% | G449G (GGT→GGC) | ggt → | gamma‑glutamyltranspeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,641,712 | 0 | T | C | 68.1% | 17.5 / 8.4 | 25 | G449G (GGT→GGC) | ggt | gamma‑glutamyltranspeptidase |
| Reads supporting (aligned to +/- strand): ref base T (4/4); new base C (7/10); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGT > minE/2641646‑2641774 | taataaCCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGa < 1:1525312/69‑1 (MQ=255)taataaCCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGa < 1:2848065/69‑1 (MQ=255) ggatgATTTCTCCGCAAAACCAGGCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACTCCGt < 1:1468955/69‑1 (MQ=255) ggatgATTTCTCCGCAAAACCAGGCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGt < 1:1744635/69‑1 (MQ=255) ggatgATTTCTCCGCAAAACCAGGCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGt < 1:1801699/69‑1 (MQ=255) gatgatTTCTCCGCAAAACCAGGCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGt < 1:932415/68‑1 (MQ=255) gatgatTTCTCCGCAAAACCAGGCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGt < 1:1500963/68‑1 (MQ=255) gatgatTTCTCCGCAAAACCAGGCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGt < 1:79439/68‑1 (MQ=255) gatgatTTCTCCGCAAAACCAGGCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGt < 1:2283120/68‑1 (MQ=255) atgatTTCTCCGCAAAACCAGGCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGt < 1:3086870/67‑1 (MQ=255) ttgtcCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCg > 1:3196921/4‑69 (MQ=255) ggCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGt < 1:59302/47‑1 (MQ=255) gCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGcc > 1:3251498/1‑44 (MQ=255) gCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGTCGGGCCGAATAAACGCCCGCTgt > 1:2839837/1‑69 (MQ=255) gCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGTCGGGCCGAATAAACGCCCGCTgt > 1:332240/1‑69 (MQ=255) gCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGTCGGGCCGAATAAACGCCCGCTgt > 1:3255240/1‑69 (MQ=255) gCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGTCGGGCCGAATAAACGCCCGCTgt > 1:1255280/1‑69 (MQ=255) gCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGTCGGGCCGAATAAACGCCCGCTgt > 1:2609333/1‑69 (MQ=255) gCGTACCGAACGTTTACGGGCTGGTGGGCGGCGATGCCAACGCCGTCGGGCCGAATAAACGCCCGCTgt > 1:2539736/1‑69 (MQ=255) ccGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCTAACAAACGCCCGCTgtcgtc < 1:1414432/68‑1 (MQ=255) ccGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTgtcgtc < 1:3397510/68‑1 (MQ=255) gTTTACGGGCTGGTGGGCGGCGATGCCAACGCCgg < 1:1733124/35‑2 (MQ=37) ggCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTgtggt > 1:668902/1‑68 (MQ=255) ggCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTgtggt > 1:70734/1‑68 (MQ=255) ggCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTgtggt > 1:1658925/1‑68 (MQ=255) | TAATAACCAGATGGATGATTTCTCCGCCAAACCGGGCGTACCGAACGTTTACGGGCTGGTGGGCGGTGATGCCAACGCCGTCGGGCCGAACAAACGCCCGCTGTCGTCGATGTCGCCGACCATTGTGGT > minE/2641646‑2641774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |