| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,641,872 | T→A | 78.6% | L503M (TTG→ATG) | ggt → | gamma‑glutamyltranspeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,641,872 | 0 | T | A | 78.6% | 95.0 / 21.5 | 56 | L503M (TTG→ATG) | ggt | gamma‑glutamyltranspeptidase |
| Reads supporting (aligned to +/- strand): ref base T (4/8); new base A (36/8); total (40/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
GCCCAGGCGGTAGCCGGATCATCACTACAGTGCTGCAAATGGTGGTGAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGACCAATGCGCCGCGTTTCCACCATCAGTGGTTGCCG > minE/2641807‑2641925 | gCCCAGGCGGTAGCCGGATCATCACTACAGTGCTGCAAATGGTGGTGAATAGCATCGATTATGGCTTGa < 1:3216488/69‑1 (MQ=255)gCCCAGGCGGTAGCCGGATCATCACTACAGTGCTGCAAATGGTGGTGAATAGCATCGATTATGGCTTGa < 1:3365212/69‑1 (MQ=255)gCCCAGGCGGTAGCCGGATCATCACTACAGTGCTGCAAATGGTGGTGAATAGCATCGATTATGGCTTGa < 1:376167/69‑1 (MQ=255) ccAGGCGGTAGCCGGATCATCACTACAGTGCTGCAAATGGTGGTGAATAGCATCGATTATGGCTTGa < 1:2421297/67‑1 (MQ=255) atcatcACTACAGTGCTGCAAATGGTGGTGAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGAcc < 1:737827/69‑1 (MQ=255) gTGCTGCAAATGGTGGTGAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGACCAATGCGCCGCGt > 1:1121149/1‑69 (MQ=255) gTGCTGCAAATGGTGGTGAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGACCAATGCGCCGCGt > 1:272073/1‑69 (MQ=255) gcAAATGGTGGTGAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGACCAATGCGCCGCGTTTcc > 1:2309426/1‑68 (MQ=255) aaTGGTGGTGAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGACCAATGCGCCGCGTTTccac < 1:65074/67‑1 (MQ=255) aaTGGTGGTGAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGACCAATGCGCCGCGTTTccac < 1:314797/67‑1 (MQ=255) aaTGGTGGTGAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGACCAATGCGCCGAGTTTccac < 1:2739462/67‑1 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:470842/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:524463/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:321603/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:3113789/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:3012484/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:733916/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:918559/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:234870/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:1109430/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:161722/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:1076336/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:1389694/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:1401091/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:149337/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:1591894/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:1740706/1‑69 (MQ=255) gtggtgAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:1884114/1‑69 (MQ=255) gtggtcAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:619356/1‑69 (MQ=255) gtggtcAACAGCATCAATTGTGGAATGAATGTTGCCGAAGCGACCAATGCGCCTCGTTTCCACCATCAg > 1:615464/1‑69 (MQ=255) gAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGACCAATGCGCCGCGTTTCCACCATCAGTGGtt > 1:2272618/1‑69 (MQ=255) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:3135673/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:62628/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:618633/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:2150517/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:1398318/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:1546429/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:1170714/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:1162627/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:3083705/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:1652224/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:19871/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:2600870/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:2591087/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:2451074/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:2500606/1‑40 (MQ=38) aTAGCATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:2467323/1‑40 (MQ=38) aTAGAATCGATTATGGAATGAACGTCGCCGAAGCGACCaa > 1:2448372/1‑40 (MQ=37) aGCATCGATTATGGAATGAACGTCGCCGAAGCGACCAATGCGCCGCATTTCCACCATCAGTGGTTGCCg < 1:2076706/69‑1 (MQ=255) aGCATCGATTATGGAATGAACGTCGCCGAAGCGACCAATGCGCCGCATTTCCACCATCAGTGGTTGCCg < 1:723106/69‑1 (MQ=255) aGCATCGATTATGGAATGAACGTCGCCGAAGCGACCAATGCGCCGCATTTCCACCATCAGTGGTTGCCg < 1:123148/69‑1 (MQ=255) aGCATCGATTATGGAATGAACGTCGCCGAAGCGACCAATGCGCCGCATTTCCACCATCAGTGGTTGCCg < 1:1384427/69‑1 (MQ=255) aGCATCGATTATGGAATGAACGTCGCCGAAGCGACCAATGCGCCGCATTTCCACCATCAGTGGTTGCCg < 1:2022315/69‑1 (MQ=255) aGCATCGATTATGGAATGAACGTCGCCGAAGCGACCAATGCGCCGCATTTCCACCATCAGTGGTTGCCg < 1:1992684/69‑1 (MQ=255) aGCATCGATTATGGAATGAACGTCGCCGAAGCGACCAATGCGCCGCATTTCCACCATCAGTGGTTGCCg < 1:2061700/69‑1 (MQ=255) gCATCGATTATGGAATGAACGTCGCCGAAGCGACCAATGCGCCGCATTTCCACCATCAGTGGTTGCCg < 1:2562852/68‑1 (MQ=255) | GCCCAGGCGGTAGCCGGATCATCACTACAGTGCTGCAAATGGTGGTGAATAGCATCGATTATGGCTTGAACGTCGCCGAAGCGACCAATGCGCCGCGTTTCCACCATCAGTGGTTGCCG > minE/2641807‑2641925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |