Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,643,235 C→T 23.5% S289S (AGC→AGT gntR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,643,2350CT23.5% 129.7 / 29.8 97S289S (AGC→AGTgntRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (64/10);  new base T (17/6);  total (81/16)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

ATGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGGCGCGTATTCGT  >  minE/2643173‑2643298
                                                              |                                                               
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCt                                                            >  1:947489/1‑68 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCt                                                            >  1:1317425/1‑68 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:990714/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:1694189/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:1833459/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:192781/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:2462909/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:2788123/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:1384378/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:3112810/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:3292374/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:476460/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:94004/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:502155/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:746865/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:658471/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:662445/1‑69 (MQ=255)
atGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGCAGCCACGACTTGCGAGCGTGCTg                                                           >  1:911245/1‑69 (MQ=255)
 tGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTg                                                           >  1:3016069/1‑68 (MQ=255)
 tGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGa                                                          >  1:3265867/1‑69 (MQ=255)
 tGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGa                                                          >  1:2637344/1‑69 (MQ=255)
 tGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGa                                                          >  1:1405387/1‑69 (MQ=255)
 tGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGa                                                          >  1:1194849/1‑69 (MQ=255)
 tGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGa                                                          >  1:1345610/1‑69 (MQ=255)
 tGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGa                                                          >  1:3243700/1‑69 (MQ=255)
 tGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGACACGACTTGCGAGCGTGCTGa                                                          >  1:2550191/1‑69 (MQ=255)
   gCGATTGCTGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGAc                                                         >  1:1835586/1‑68 (MQ=255)
   gCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGAcg                                                        >  1:2859059/1‑69 (MQ=255)
   gCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGAcg                                                        >  1:1987640/1‑69 (MQ=255)
   gCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGAcg                                                        >  1:1012887/1‑69 (MQ=255)
   gCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGAcg                                                        >  1:1542267/1‑69 (MQ=255)
   gCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGAcg                                                        >  1:1367763/1‑69 (MQ=255)
         gCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGAcgccgc                                                    >  1:1313419/1‑67 (MQ=255)
         gCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGAcgccgc                                                    >  1:2757157/1‑67 (MQ=255)
              tttCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             <  1:2990923/69‑1 (MQ=255)
              tttCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             <  1:1524025/69‑1 (MQ=255)
               ttCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCgg                                            >  1:3160522/1‑69 (MQ=255)
               ttCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCgg                                            >  1:261935/1‑69 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGc                                              >  1:858166/1‑60 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             >  1:1724779/1‑61 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             >  1:1000795/1‑61 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             >  1:949166/1‑61 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             >  1:1070188/1‑61 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             >  1:1286013/1‑61 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             >  1:2984798/1‑61 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             >  1:2540857/1‑61 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             >  1:2088768/1‑61 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCg                                             >  1:1674980/1‑61 (MQ=255)
                      gTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGc                                      >  1:1769220/1‑68 (MQ=255)
                       tCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAg                                    <  1:724212/69‑1 (MQ=255)
                       tCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAg                                    <  1:1134023/69‑1 (MQ=255)
                       tCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAg                                    <  1:1536080/69‑1 (MQ=255)
                       tCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAg                                    <  1:3078025/69‑1 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTg                                                 >  1:1266263/1‑55 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTTAGCGGATGGGCAgc                                   >  1:1858952/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:877554/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:782231/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:1190770/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:119979/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:1217081/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:3346724/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:1564879/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:1372677/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:1529842/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:2728846/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:2354654/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:253562/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:142676/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAgc                                   >  1:211411/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGGCTAGCGAGTGTGCTGACGCCGCGTGAGCGGATGGGCAg                                    >  1:3206533/1‑68 (MQ=255)
                        cATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAg                                    <  1:1022789/68‑1 (MQ=255)
                            aCATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTAtt                                >  1:2817219/1‑68 (MQ=255)
                            aCATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTg                               >  1:3291843/1‑69 (MQ=255)
                            aCATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTg                               >  1:2128549/1‑69 (MQ=255)
                            aCATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTg                               >  1:2756541/1‑69 (MQ=255)
                            aCATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTg                               >  1:2451265/1‑69 (MQ=255)
                            aCATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTg                               >  1:1038837/1‑69 (MQ=255)
                            aCATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTg                               >  1:289768/1‑69 (MQ=255)
                                tGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGgcg                            >  1:264079/1‑68 (MQ=255)
                                               gCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGgc            >  1:2072902/1‑69 (MQ=255)
                                               gCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGgc            >  1:750076/1‑69 (MQ=255)
                                               gCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGgc            >  1:1738030/1‑69 (MQ=255)
                                               gCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGgc            >  1:486432/1‑69 (MQ=255)
                                               gCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGgc            >  1:450925/1‑69 (MQ=255)
                                               gCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGgc            >  1:1400425/1‑69 (MQ=255)
                                               gCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGgc            >  1:2044754/1‑69 (MQ=255)
                                               gCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGgc            >  1:2884004/1‑69 (MQ=255)
                                                   cGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGt                                   <  1:659653/42‑1 (MQ=255)
                                                    gACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGGCGCGTa       >  1:1686630/1‑69 (MQ=255)
                                                      cTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGGCGCGTAtt     <  1:1500680/69‑1 (MQ=255)
                                                      cTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGGCGCGTAtt     <  1:2750548/69‑1 (MQ=255)
                                                           gAGTGTGCTGACGTCGCGTGAGCGGATGGGCAGCATTGGCGCTGAGCGCCTGCTGGCGCGTATTCGt  <  1:2131160/67‑1 (MQ=255)
                                                           gAGTGTGCTGACGCCGCGTGAGCGGATGGGCAGCATTGGCGCTGAGCGCCTGCTGGCGCGTATTCGt  <  1:67451/67‑1 (MQ=255)
                                                           gAGTGTGCTGACGCCGCGTGAGCGGATGGGCAGCATTGGCGCTGAGCGCCTGCTGGCGCGTATTCGt  <  1:693394/67‑1 (MQ=255)
                                                           gAGTGTGCTGACGCCGCGTGAGCGGATGGGCAGCATTGGCGCTGAGCGCCTGCTGGCGCGTATTCGt  <  1:699286/67‑1 (MQ=255)
                                                           gAGTGTGCTGACGCCGCGTGAGCGGATGGGCAGCATTGGCGCTGAGCGCCTGCTGGCGCGTATTCGt  <  1:1126509/67‑1 (MQ=255)
                                                           gAGTGTGCTGACGCCGCGTGAGCGGATGGGCAGCATTGGCGCTGAGCGCCTGCTGGCGCGTATTCGt  <  1:2931268/67‑1 (MQ=255)
                                                              |                                                               
ATGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGCGTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGGCGCGTATTCGT  >  minE/2643173‑2643298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: