| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,645,878 | C→A | 29.2% | L49L (CTG→CTT) | yhgN ← | predicted antibiotic transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,645,878 | 0 | C | A | 29.2% | 88.8 / 35.4 | 65 | L49L (CTG→CTT) | yhgN | predicted antibiotic transporter |
| Reads supporting (aligned to +/- strand): ref base C (3/43); new base A (3/16); total (6/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01 | |||||||||||
CTAAGAAATGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTATGTTTCAGT > minE/2645828‑2645944 | cTAAGAAATGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAAc < 1:2870715/69‑1 (MQ=255) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:1803429/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:2255677/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:525392/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:2151246/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:2417073/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:521450/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:1003002/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:867879/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:375713/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:1763779/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:1560081/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:369667/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:347122/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:3023577/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:1117783/51‑1 (MQ=38) aaGAAATGCCAGAATTTTCTCGCCCGCAAACAGGAACACCAGCATCACAAg < 1:3059658/51‑1 (MQ=38) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCTCAc < 1:1588184/69‑1 (MQ=255) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCAc > 1:1053818/1‑69 (MQ=255) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCAc < 1:2722648/69‑1 (MQ=255) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCAc < 1:2536556/69‑1 (MQ=255) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCAc < 1:2329313/69‑1 (MQ=255) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCAc > 1:231812/1‑69 (MQ=255) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCAc < 1:3398187/69‑1 (MQ=255) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCAc < 1:2121346/69‑1 (MQ=255) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCAc < 1:2125378/69‑1 (MQ=255) tGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCAc < 1:973679/69‑1 (MQ=255) ggAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:2058965/68‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:3323271/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:3101180/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:3406497/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:1214504/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:2964101/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:872645/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:1632783/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:1764084/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:868751/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:1897031/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:2648695/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:2627273/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:623404/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:606147/69‑1 (MQ=255) aGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:244963/69‑1 (MQ=255) gAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATg < 1:3195410/68‑1 (MQ=255) acagCAGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAg > 1:976148/1‑69 (MQ=255) acacCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAg < 1:2714924/69‑1 (MQ=255) acacCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAg < 1:106236/69‑1 (MQ=255) acacCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAg < 1:1300576/69‑1 (MQ=255) acacCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAg < 1:2984691/69‑1 (MQ=255) acacCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAg < 1:2618057/69‑1 (MQ=255) acacCAGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAg > 1:2207607/1‑69 (MQ=255) acacCAGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAg > 1:2015742/1‑69 (MQ=255) acCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTa < 1:3350615/69‑1 (MQ=255) acCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTa < 1:1242653/69‑1 (MQ=255) acCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTa < 1:1809248/69‑1 (MQ=255) acCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTa < 1:1826556/69‑1 (MQ=255) acCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTa < 1:676515/69‑1 (MQ=255) acCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTa < 1:671975/69‑1 (MQ=255) acCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTa < 1:2832863/69‑1 (MQ=255) acCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTa < 1:289999/69‑1 (MQ=255) acCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTa < 1:3289122/69‑1 (MQ=255) aGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTATGt < 1:1975825/69‑1 (MQ=255) aGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTATGt < 1:3050767/69‑1 (MQ=255) aGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTATGt < 1:3128129/69‑1 (MQ=255) aCCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTATGTTTCAGt > 1:978037/1‑69 (MQ=255) | CTAAGAAATGCCAGAATTTTCTCACCCGCAAACAGGAACACCAGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTCAGTATGTTTCAGT > minE/2645828‑2645944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |