| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,647,443 | T→G | 15.0% | intergenic (+123/‑150) | asd → / → glgB | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,647,443 | 0 | T | G | 15.0% | 220.1 / 29.8 | 113 | intergenic (+123/‑150) | asd/glgB | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme |
| Reads supporting (aligned to +/- strand): ref base T (51/45); new base G (7/10); total (58/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
AGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGC > minE/2647380‑2647507 | aGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATa > 1:980173/1‑69 (MQ=255)aGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATa > 1:749196/1‑69 (MQ=255)aGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATa > 1:357030/1‑69 (MQ=255)aGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATa > 1:3250644/1‑69 (MQ=255)aGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATa > 1:2399360/1‑69 (MQ=255)aGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATa > 1:2367653/1‑69 (MQ=255)aGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATa > 1:2313565/1‑69 (MQ=255)aGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATa > 1:1687917/1‑69 (MQ=255)aGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAACGGTGGCTGAATa > 1:1392643/1‑69 (MQ=255) cAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATg < 1:2430463/69‑1 (MQ=255) cAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATg < 1:578465/69‑1 (MQ=255) cAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATg < 1:548393/69‑1 (MQ=255) cAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATg < 1:2773068/69‑1 (MQ=255) cAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATg < 1:1997096/69‑1 (MQ=255) cAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATg < 1:1658248/69‑1 (MQ=255) cAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATg < 1:137789/69‑1 (MQ=255) aGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATg < 1:2287680/68‑1 (MQ=255) tGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTa < 1:1919283/69‑1 (MQ=255) tGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTa < 1:2388023/69‑1 (MQ=255) tCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTAtt > 1:741135/1‑67 (MQ=255) tCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTAtt > 1:1410454/1‑67 (MQ=255) tCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTAtt > 1:546999/1‑67 (MQ=255) tCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTAtt > 1:505059/1‑67 (MQ=255) tCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTAtt > 1:494705/1‑67 (MQ=255) tCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTAt > 1:2980491/1‑66 (MQ=255) gATTTACCGTGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGc < 1:63403/69‑1 (MQ=255) gATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGc < 1:1693502/69‑1 (MQ=255) gATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGc < 1:2783638/69‑1 (MQ=255) gATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGc < 1:189035/69‑1 (MQ=255) tttACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCt > 1:1511177/1‑69 (MQ=255) tttACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCt > 1:2607754/1‑69 (MQ=255) tttACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCt > 1:1226141/1‑69 (MQ=255) ttACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt < 1:3124220/69‑1 (MQ=255) ttACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt < 1:1068668/69‑1 (MQ=255) tACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt < 1:477158/68‑1 (MQ=255) gggAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTg < 1:2502947/69‑1 (MQ=255) gggAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTg < 1:118461/69‑1 (MQ=255) gggAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTg < 1:866998/69‑1 (MQ=255) tAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGa > 1:2151902/1‑60 (MQ=255) tAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGgac > 1:1214709/1‑69 (MQ=255) tAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGgac > 1:2035908/1‑69 (MQ=255) tAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGgac > 1:1707256/1‑69 (MQ=255) tAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGgac > 1:2224077/1‑69 (MQ=255) tAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGgac > 1:29352/1‑69 (MQ=255) tAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGgac > 1:1599283/1‑69 (MQ=255) tGGCTATTCTTTAAGGGTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCa > 1:3321864/1‑67 (MQ=255) tGGCTATTCTTTAAGGGTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCa > 1:272010/1‑67 (MQ=255) tGGCTATTCTTTAAGGGTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCa > 1:2243395/1‑67 (MQ=255) tGGCTATTCTTTAAGGGTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCa > 1:3283615/1‑67 (MQ=255) tGGCTATTCTTTAAGGGTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCa > 1:542727/1‑67 (MQ=255) tGGCTATTCTTTAAGGGTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCa > 1:1525475/1‑67 (MQ=255) tGGCTATTCTTTAAGGGTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCa > 1:1158496/1‑67 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagag > 1:2121939/1‑69 (MQ=255) cTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGc < 1:1386743/40‑1 (MQ=255) ttCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGa > 1:3233670/1‑68 (MQ=255) ttCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGa > 1:2720662/1‑68 (MQ=255) ttCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGa > 1:1518100/1‑68 (MQ=255) ttCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGa > 1:2485125/1‑68 (MQ=255) tCTTTAAGGGTGGCTGAATACATGAGTATTCACAGc < 1:401763/36‑1 (MQ=255) ctgtAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:1465274/4‑64 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt > 1:1476779/1‑38 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt > 1:650529/1‑38 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt > 1:305415/1‑38 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt > 1:223759/1‑38 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt > 1:2184567/1‑38 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:2466199/1‑64 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:76871/1‑64 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:1551166/1‑64 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:685394/64‑1 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:1185947/64‑1 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:1681289/1‑64 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:524567/1‑64 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:1872992/1‑64 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:461030/64‑1 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:1906661/64‑1 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:1391759/1‑64 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:1218048/64‑1 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:2259811/1‑64 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag > 1:2978180/1‑64 (MQ=255) aGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:408580/67‑1 (MQ=255) aGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:2870167/67‑1 (MQ=255) aGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:1420721/67‑1 (MQ=255) aGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:161673/67‑1 (MQ=255) aGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGcaca < 1:624999/67‑1 (MQ=255) ggTGGGGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACagag < 1:2978625/68‑1 (MQ=255) ggTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACagag < 1:2294250/68‑1 (MQ=255) ggTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACagag < 1:2750321/68‑1 (MQ=255) ggTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACagag < 1:8437/68‑1 (MQ=255) ggTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACagag < 1:817173/68‑1 (MQ=255) ggTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACagag < 1:1189603/68‑1 (MQ=255) ggTGGCGGAATACACGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACagag < 1:2744820/68‑1 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:3257593/56‑1 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:3091221/56‑1 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:1400197/56‑1 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:3097602/56‑1 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:1726701/56‑1 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:321531/56‑1 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCagagag < 1:2718412/56‑1 (MQ=255) tGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgc > 1:1320555/1‑69 (MQ=255) tGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgc > 1:1747538/1‑69 (MQ=255) | AGCGCAGATGTTTCATGATTTACCGGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGC > minE/2647380‑2647507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |