| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,647,952 | C→T | 55.8% | R120R (CGC→CGT) | glgB → | 1,4‑alpha‑glucan branching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,647,952 | 0 | C | T | 55.8% | 6.8 / 39.6 | 52 | R120R (CGC→CGT) | glgB | 1,4‑alpha‑glucan branching enzyme |
| Reads supporting (aligned to +/- strand): ref base C (11/12); new base T (12/17); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01 | |||||||||||
CTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCAGATACTATGGATGG > minE/2647902‑2647993 | cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:874587/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:2701965/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:2723883/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:2788348/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:2473800/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:2367068/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:233051/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:2318427/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:2085944/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:1773504/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:1765614/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:3080091/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:144670/69‑1 (MQ=255)cTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:607829/69‑1 (MQ=255) tAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:1422830/68‑1 (MQ=255) tAATCCAGGAAATGGATGCATGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:2447357/68‑1 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTa > 1:670570/1‑54 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:3026503/1‑68 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:2896390/1‑68 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:2832014/1‑68 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:774941/1‑68 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:2713325/1‑68 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:995189/1‑68 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:2595551/1‑68 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:1418561/1‑68 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:1964427/1‑68 (MQ=255) aaTCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc > 1:768225/1‑67 (MQ=255) aaTCCAAGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgcg > 1:439455/1‑68 (MQ=255) cAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCa > 1:2606368/1‑68 (MQ=255) cAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCa > 1:1395116/1‑68 (MQ=255) cAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCa > 1:3097030/1‑68 (MQ=255) cAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCa > 1:1419273/1‑68 (MQ=255) cAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCa > 1:3432582/1‑68 (MQ=255) cAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTAACCTGCGCCAGTATGaaa > 1:637500/1‑55 (MQ=255) gAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCa < 1:2543268/69‑1 (MQ=255) gAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCa < 1:2892344/69‑1 (MQ=255) gAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCa < 1:2045338/69‑1 (MQ=255) gAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCa < 1:1706663/69‑1 (MQ=255) ggATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCAGATAc < 1:3236288/69‑1 (MQ=255) ggATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCAGATAc < 1:2644119/69‑1 (MQ=255) ggATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCAGATAc < 1:253830/69‑1 (MQ=255) tGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGa > 1:2944204/1‑38 (MQ=255) tGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGgcgcg > 1:889498/1‑51 (MQ=255) tGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGgcgcg > 1:1075233/1‑51 (MQ=255) gCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCAGATACTatggatgg > 1:5806/1‑69 (MQ=255) gCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGAGCGCATGCAGATACTatggatgg > 1:721558/1‑69 (MQ=255) atCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCa < 1:3086265/49‑1 (MQ=255) atCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCa < 1:751310/49‑1 (MQ=255) atCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCa < 1:2838652/49‑1 (MQ=255) atCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCa < 1:1329120/49‑1 (MQ=255) tGAAGGTACTCACCTACGTCCGTATGAAACCTTAGgc < 1:955417/37‑1 (MQ=255) gTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCAGATAc < 1:3107282/46‑1 (MQ=255) | CTAATCCAGGAAATGGATGCCTGGCTATTATCTGAAGGTACTCACCTGCGCCCGTATGAAACCTTAGGCGCGCATGCAGATACTATGGATGG > minE/2647902‑2647993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |