| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 227,362 | A→G | 42.0% | E52E (GAA→GAG) | crl → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 227,362 | 0 | A | G | 42.0% | 24.0 / 60.1 | 81 | E52E (GAA→GAG) | crl | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (26/21); new base G (16/18); total (42/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 | |||||||||||
ATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGG > minE/227295‑227427 | aTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAt < 1:1571511/69‑1 (MQ=255) atggATTCTTTTTCGATTGTCTGGTTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt > 1:2555481/4‑69 (MQ=255) aTCGATTCTTTTTCGATTGTCTGGCTGTATGCTTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt > 1:389898/1‑69 (MQ=255) aTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt > 1:451039/1‑69 (MQ=255) aTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt > 1:2552356/1‑69 (MQ=255) aTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt > 1:2989875/1‑69 (MQ=255) aTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt > 1:2131283/1‑69 (MQ=255) aTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt > 1:1799111/1‑69 (MQ=255) aTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt > 1:1129195/1‑69 (MQ=255) tCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg < 1:1068272/67‑1 (MQ=255) tCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg < 1:2289991/67‑1 (MQ=255) tCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg < 1:1641592/67‑1 (MQ=255) ttGTCTTGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgga < 1:1811318/69‑1 (MQ=255) ttGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgga < 1:738749/69‑1 (MQ=255) ttGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgga < 1:1215797/69‑1 (MQ=255) tATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcgc > 1:2483493/1‑69 (MQ=255) tATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcgc > 1:298397/1‑69 (MQ=255) tATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcgc > 1:2181272/1‑69 (MQ=255) tATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcgc > 1:3296506/1‑69 (MQ=255) tGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa > 1:1271495/1‑68 (MQ=255) cGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg < 1:1776117/53‑1 (MQ=255) cGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg < 1:2531238/53‑1 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:283457/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:1713907/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:1052615/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:3106096/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:3117260/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:2411687/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:827852/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:679912/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:2241137/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:2170287/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:1450043/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:2941421/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:2718641/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:2883406/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:610949/1‑44 (MQ=255) gTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCtg > 1:478829/1‑44 (MQ=255) gTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATccc > 1:2397004/1‑67 (MQ=255) gTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt > 1:279390/1‑69 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:3078877/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:3007118/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:3237510/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:3316211/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:3402895/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:377453/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:92878/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:970739/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:1041710/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:2791039/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:1281386/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:2690716/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:2653232/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:2649115/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:2525230/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:2435476/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:2351171/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAGTTCTGGGGCTGGtgga < 1:2276442/35‑1 (MQ=255) ggCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa < 1:1300738/69‑1 (MQ=255) gAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc < 1:3310863/69‑1 (MQ=255) gAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc < 1:1587239/69‑1 (MQ=255) gAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc < 1:570576/69‑1 (MQ=255) gAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc < 1:524324/69‑1 (MQ=255) gAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc < 1:2064649/69‑1 (MQ=255) gAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc < 1:2114976/69‑1 (MQ=255) gAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc < 1:280115/69‑1 (MQ=255) gAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc < 1:3299340/69‑1 (MQ=255) gAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc < 1:3183853/69‑1 (MQ=255) gCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAcc < 1:3261713/55‑1 (MQ=255) gCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAcc < 1:1337488/55‑1 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:2151017/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:2098818/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:436384/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:1822265/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:1751002/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:256018/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:2902190/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:2719136/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:127995/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg > 1:1029326/1‑69 (MQ=255) tGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg > 1:2715369/1‑68 (MQ=255) | ATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGG > minE/227295‑227427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |