| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,649,084 | G→A | 36.6% | V498I (GTT→ATT) | glgB → | 1,4‑alpha‑glucan branching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,649,084 | 0 | G | A | 36.6% | 71.1 / 66.7 | 109 | V498I (GTT→ATT) | glgB | 1,4‑alpha‑glucan branching enzyme |
| Reads supporting (aligned to +/- strand): ref base G (42/27); new base A (16/24); total (58/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
TGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACTGAAAACTTCG > minE/2649016‑2649150 | tGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCgg > 1:2972635/1‑69 (MQ=255)tGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCgg > 1:375324/1‑69 (MQ=255)tGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCgg < 1:754798/69‑1 (MQ=255)tGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCgg > 1:3304981/1‑69 (MQ=255)tGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCgg > 1:3386218/1‑69 (MQ=255)tGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCgg > 1:1541771/1‑69 (MQ=255) gCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGCCACCCTGGACTACATGAAGCTCGACCCGGttt > 1:2487968/1‑69 (MQ=255) gCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGttt > 1:2772190/1‑69 (MQ=255) aCAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAg < 1:1406826/68‑1 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACCCCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatc > 1:2070044/1‑69 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCgg > 1:3090772/1‑54 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCg > 1:212545/1‑61 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGt > 1:1951262/1‑66 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGt > 1:2659572/1‑66 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGt > 1:2803999/1‑66 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGt > 1:2655599/1‑66 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGt > 1:87133/1‑66 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGt > 1:1025251/1‑66 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatc > 1:2903591/1‑69 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatc > 1:698182/1‑69 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatc > 1:727626/1‑69 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTat < 1:206622/68‑1 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTat < 1:2199516/68‑1 (MQ=255) ggAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatcatc > 1:1670853/1‑69 (MQ=255) ggAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatcatc > 1:2788351/1‑69 (MQ=255) ggAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatcatc > 1:179437/1‑69 (MQ=255) tCGGCTGGATGCATGACCCCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGCTa > 1:636147/1‑69 (MQ=255) tCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa > 1:340576/1‑69 (MQ=255) tCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa > 1:2964352/1‑69 (MQ=255) tCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa > 1:1678370/1‑69 (MQ=255) tCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa > 1:1313851/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTg > 1:971896/1‑68 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:9814/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:3408114/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:3417824/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:463062/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:46442/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:565166/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:63031/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:963739/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:105939/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:1810558/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:1954395/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:1378126/1‑69 (MQ=255) ggATGCATGACACCCTGGACTACATGAAGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGa > 1:1657572/1‑69 (MQ=255) tGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCgg < 1:1913207/69‑1 (MQ=255) tGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCgg < 1:3136644/69‑1 (MQ=255) tGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCgg < 1:3160742/69‑1 (MQ=255) tGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCgg < 1:3195489/69‑1 (MQ=255) gACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCgg < 1:3293246/68‑1 (MQ=255) acacCCTGGACTACATGAAGCTCTACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGa > 1:1808519/1‑69 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCggg > 1:742877/1‑68 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCggg > 1:2404042/1‑68 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGa > 1:729966/1‑69 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGa > 1:2365554/1‑69 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATAATCACGATAAACTGACCTTCggg > 1:3312495/1‑68 (MQ=255) cTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTAc < 1:1085295/67‑1 (MQ=255) cTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTAc < 1:100879/67‑1 (MQ=255) cTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTAc < 1:2130686/67‑1 (MQ=255) cTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACa > 1:1215160/1‑68 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtctc < 1:713484/61‑1 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtctc < 1:1413567/61‑1 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtctc < 1:2583987/61‑1 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtctc < 1:2146677/61‑1 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtctc < 1:3075850/61‑1 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTa > 1:3186770/1‑69 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTa > 1:2676881/1‑69 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTa > 1:1856261/1‑69 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTa > 1:63419/1‑69 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTa > 1:253629/1‑69 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTa > 1:1657735/1‑69 (MQ=255) aTGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa > 1:424105/1‑40 (MQ=255) aaGCTCTACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:941852/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:127253/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:234052/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:624916/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:1302546/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:127798/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:671302/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:1066328/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:2360292/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:1593221/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:2363514/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:1540802/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:2904281/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:1934525/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:1963122/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:3160810/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:2014989/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:3399767/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:1674260/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:2883809/69‑1 (MQ=255) aaGCTCGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:2533891/69‑1 (MQ=255) tCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACTGaaa < 1:1724398/69‑1 (MQ=255) tCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACTGaaa < 1:3241997/69‑1 (MQ=255) tCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACTGaaa < 1:350367/69‑1 (MQ=255) tCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACTGaaa < 1:2843199/69‑1 (MQ=255) tCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACTGaaa < 1:1817988/69‑1 (MQ=255) cGACCCGATTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATGCTCTACAACTACACt < 1:1019850/64‑1 (MQ=255) cGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACTGAAAACTTCg < 1:2486501/69‑1 (MQ=255) | TGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACTGAAAACTTCG > minE/2649016‑2649150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |