| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,652,018 | T→C | 56.1% | I84I (ATT→ATC) | glgC → | glucose‑1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,652,018 | 0 | T | C | 56.1% | 16.7 / 49.2 | 66 | I84I (ATT→ATC) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (16/13); new base C (31/6); total (47/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-01 | |||||||||||
AACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAG > minE/2651953‑2652086 | aaCTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAg > 1:696793/1‑69 (MQ=255)aaCTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAg > 1:1826941/1‑69 (MQ=255)aaCTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAg > 1:2309937/1‑69 (MQ=255) cgtcgtATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGtaa < 1:2428650/69‑3 (MQ=255) cgtcgtATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCa < 1:1756374/69‑1 (MQ=255) aTCACCGAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:439251/1‑69 (MQ=255) aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:1116377/1‑69 (MQ=255) aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:3235705/1‑69 (MQ=255) aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:3185006/1‑69 (MQ=255) aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:2469215/1‑69 (MQ=255) aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaagaa > 1:1693368/1‑69 (MQ=255) aTCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATgaaga > 1:970537/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2918498/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2152700/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2181579/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2170270/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:110235/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2927282/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2932503/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:2936141/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:3054695/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:3135469/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:3220804/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:383208/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:5143/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1649043/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1590369/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1782094/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1956797/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1137480/1‑68 (MQ=255) cACCCAGTACCAGTCCCACACTCTGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGaaa > 1:1423511/1‑68 (MQ=255) gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:2161598/69‑1 (MQ=255) gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:305244/69‑1 (MQ=255) gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:2947770/69‑1 (MQ=255) gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:347598/69‑1 (MQ=255) gTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:2079395/69‑1 (MQ=255) gTACCAGTCCCACACTCTGGTGCAGCACATTAAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:1247316/69‑1 (MQ=255) taacAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAg < 1:2412654/66‑1 (MQ=255) cccACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCg < 1:478741/69‑1 (MQ=255) cccACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCg > 1:811799/1‑69 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1338156/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:527759/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:3375761/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:720599/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1531278/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:776671/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:105928/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1890125/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1776291/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:1843615/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:227564/1‑35 (MQ=255) acacTCTGGTGCAGCACATCCAGCGCGGCTGGTCa > 1:2693298/1‑35 (MQ=255) ctctGGTGCAGTACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:1387822/69‑1 (MQ=255) ctctGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc > 1:321469/1‑69 (MQ=255) ctctGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:407206/69‑1 (MQ=255) ctctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:2950935/69‑1 (MQ=255) ctctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:1711543/69‑1 (MQ=255) ctctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:814019/69‑1 (MQ=255) ctctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:2884190/69‑1 (MQ=255) tctGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgct < 1:3407668/69‑1 (MQ=255) tctGGTGCAGCACATCCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATctgc < 1:606070/68‑1 (MQ=255) cagcaCATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGa < 1:2460901/47‑1 (MQ=255) cagcaCATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCcag > 1:345245/1‑67 (MQ=255) cagcaCATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCcag > 1:3149976/1‑67 (MQ=255) acaTTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCcagcac > 1:2693155/1‑66 (MQ=255) caTTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCcagcacag > 1:2031106/1‑67 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:2113214/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:811135/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:2281354/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:2355651/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:670894/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:1288320/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:381485/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:2847559/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:3244031/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:3150704/69‑1 (MQ=255) tCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCagag < 1:1691259/69‑1 (MQ=255) | AACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTCTGGTGCAGCACATTCAGCGCGGCTGGTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAG > minE/2651953‑2652086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |