| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 227,492 | C→T | 24.8% | L96L (CTG→TTG) | crl → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 227,492 | 0 | C | T | 24.8% | 84.0 / 21.6 | 68 | L96L (CTG→TTG) | crl | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (45/6); new base T (11/6); total (56/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01 | |||||||||||
CTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAG > minE/227429‑227557 | cTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAg < 1:1503086/69‑1 (MQ=255)cTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAg < 1:776016/69‑1 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:436736/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:2675771/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:2408050/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:2339950/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:3212993/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:3296124/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:3300774/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:1369746/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:1365869/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:1279759/1‑69 (MQ=255) gATAAAGCAGGCGACTGGACGAGTGTTCGGATAAAAGATGCCGAAGTGGTTGAACGATTGGAGCACAcc > 1:914328/1‑69 (MQ=255) ggAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTc > 1:788170/1‑66 (MQ=255) ggAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTc > 1:1693349/1‑66 (MQ=255) ggAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTc > 1:2944419/1‑66 (MQ=255) ggAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTc > 1:995538/1‑66 (MQ=255) gtgtTCCGGTAAAAGACACTGAAGTGGTTGAACTACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGc > 1:1695712/1‑69 (MQ=255) gtgtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGc > 1:567151/1‑69 (MQ=255) gtgtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGc > 1:475677/1‑69 (MQ=255) gtgtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGc > 1:3023343/1‑69 (MQ=255) gtgtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGc > 1:3405390/1‑69 (MQ=255) gtgtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGc > 1:81580/1‑69 (MQ=255) gtgtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGc > 1:3170565/1‑69 (MQ=255) gtgtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGc > 1:1565863/1‑69 (MQ=255) gtgtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGc > 1:2026157/1‑69 (MQ=255) ggTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTg < 1:2716947/68‑1 (MQ=255) ggTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTg < 1:1887193/68‑1 (MQ=255) ggTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTg < 1:1046640/68‑1 (MQ=255) gTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTg < 1:2693111/67‑1 (MQ=255) aGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgct > 1:1052905/1‑69 (MQ=255) aGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgct > 1:864117/1‑69 (MQ=255) aGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgct > 1:1688300/1‑69 (MQ=255) aGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgct > 1:3434018/1‑69 (MQ=255) aGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgct > 1:31800/1‑69 (MQ=255) aGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgct > 1:3172577/1‑69 (MQ=255) aGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgct > 1:2736199/1‑69 (MQ=255) aGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgc > 1:2987621/1‑68 (MQ=255) gACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgctg > 1:303026/1‑69 (MQ=255) gACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgctg > 1:3028918/1‑69 (MQ=255) gACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgctg > 1:920498/1‑69 (MQ=255) gACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgctg > 1:2099838/1‑69 (MQ=255) gACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgctg > 1:1665575/1‑69 (MQ=255) gACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgctg > 1:481535/1‑69 (MQ=255) gACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAActgctg > 1:436515/1‑69 (MQ=255) cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:3035679/1‑69 (MQ=255) cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:2705043/1‑69 (MQ=255) cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:116082/1‑69 (MQ=255) cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:1334050/1‑69 (MQ=255) cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:1619916/1‑69 (MQ=255) cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:455622/1‑69 (MQ=255) cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:3420277/1‑69 (MQ=255) cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:2059207/1‑69 (MQ=255) cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:2958733/1‑69 (MQ=255) gAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGacgacg > 1:2585842/1‑69 (MQ=255) gAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGacgacg > 1:665101/1‑69 (MQ=255) ggTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTg > 1:706738/1‑46 (MQ=255) ggTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGaa > 1:2375904/1‑69 (MQ=255) ggTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGaa > 1:2990706/1‑69 (MQ=255) ggTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGa > 1:3228813/1‑68 (MQ=255) ggTTGAACGACTGGAGCACACCATGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGaa > 1:584141/1‑69 (MQ=255) gTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAAt > 1:1690027/1‑69 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTTCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:83764/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:596310/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:329984/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:2715066/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:2940824/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:924377/69‑1 (MQ=255) | CTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAG > minE/227429‑227557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |