| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,658,057 | A→G | 79.0% | N342N (AAT→AAC) ‡ | glpD ← | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,658,057 | 0 | A | G | 79.0% | 143.7 / 26.2 | 99 | N342N (AAT→AAC) ‡ | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (7/14); new base G (63/15); total (70/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
CCAGTTTTCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCACGGGTAATAGCCTGCGGCGAGTCGGA > minE/2657999‑2658113 | ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:2659389/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:213020/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:2001615/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:666449/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:910794/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:1036464/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:1671938/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:926592/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:155099/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:2696582/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:950092/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:3394685/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:3166099/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatc < 1:1102470/67‑1 (MQ=255)ccAGTTTGCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcata < 1:3175879/67‑2 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:472405/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:3298389/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:3296675/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:2159419/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1964135/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:2222807/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1918154/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:574463/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:418610/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1746640/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1711020/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1133574/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:2510806/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1140679/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:2604240/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1544486/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1378793/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1368968/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAg > 1:2494585/1‑68 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAg > 1:1835109/1‑68 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAg > 1:1586498/1‑68 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAg > 1:2163285/1‑68 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAg > 1:3026484/1‑68 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGCGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:379614/1‑69 (MQ=255) tAGGTGGTCAGCTTACCGCCGAATACCGACAGAAGCGGGGCTTTGCCGTCTTCATCATGGATATCAAgg > 1:1125351/1‑69 (MQ=255) ggtCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatca > 1:1078094/1‑52 (MQ=255) ggtCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatca > 1:1949790/1‑52 (MQ=255) ggtCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatca > 1:1172800/1‑52 (MQ=255) ggtCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatca > 1:3115191/1‑52 (MQ=255) ggtCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatca > 1:526664/1‑52 (MQ=255) ggtCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatca > 1:2366610/1‑52 (MQ=255) ggtCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatca > 1:3255440/1‑52 (MQ=255) ggtCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatca > 1:972509/1‑52 (MQ=255) ggtCAGCTTACCGCCGAATACCGACAGCAGCGGGGCTTTGCCGTCTtcatca > 1:2502202/1‑52 (MQ=255) aGCTTACCGCCGAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCa > 1:1990251/1‑69 (MQ=255) aGCTTACCGCCGAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCa > 1:2868041/1‑69 (MQ=255) aGCTTACCGCCGAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCa > 1:1602928/1‑69 (MQ=255) aGCTTACCGCCGAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCa > 1:2309934/1‑69 (MQ=255) aGCTTACCGCCGAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCa > 1:2291871/1‑69 (MQ=255) aGCTTACCGCCGAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCa > 1:10945/1‑69 (MQ=255) aGCTTACCGCCGAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCa > 1:631652/1‑69 (MQ=255) cgccgAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCACGGGTa < 1:1663540/68‑1 (MQ=255) gccgAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCACGGGt < 1:3268716/66‑1 (MQ=255) gccgAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCACGGGt < 1:1274967/66‑1 (MQ=255) cgAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCACGGGt < 1:929657/64‑1 (MQ=255) ccGACAGCAGCGGTGCTTTGCCATTTTCATCATGaa < 1:3143836/36‑1 (MQ=255) ccGACAGCAGCGGTGCTTTGCCATTTTCATCATGaa < 1:2215339/36‑1 (MQ=255) ccGACAGCAGCGGTGCTTTGCCATTTTCATCATGaa < 1:3220888/36‑1 (MQ=255) ccGACAGCAGCGGTGCTTTGCCATTTTCATCATGaa < 1:3211458/36‑1 (MQ=255) ccGACAGCAGCGGTGCTTTGCCATTTTCATCATGaa < 1:1756391/36‑1 (MQ=255) ccGACAGCAGCGGTGCTTTGCCATTTTCATCATGaa < 1:3058041/36‑1 (MQ=255) ccGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGgtgt < 1:509160/48‑1 (MQ=255) ccGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGgtgt < 1:2744054/48‑1 (MQ=255) ccGACAGCAGCGGCGCTTTGCCATTTTCATCATGaa < 1:559615/36‑1 (MQ=255) agcGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCACGGGTAATAGCCTGCGGCGAGTCg < 1:433878/69‑1 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCa > 1:1723387/1‑43 (MQ=25) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTgt > 1:1158781/1‑57 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCgg > 1:3086649/1‑68 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCgg > 1:2898413/1‑68 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:246470/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:981129/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:712910/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:716331/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:931341/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:3177265/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:104392/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:1152755/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:1210638/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:1497842/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:1610506/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:1654184/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:1760693/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:1804768/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:216011/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:2335860/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:2399044/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:2590577/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:2605104/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:277672/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:2943471/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:3411369/1‑69 (MQ=255) cGGGGCTTTGCCGTCTTCATCATCGATATCAAGGGTGTAATCACGGGTAATGGCCTg > 1:1996023/1‑57 (MQ=25) cGGGGCTTCGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:1965271/1‑69 (MQ=255) cGGGGCATTGCCGTCTTCATCATGGATATCAAGGGTGTAATCACGGGTAATGGCCTGTGGCGAGTCGGa > 1:442724/1‑69 (MQ=255) | CCAGTTTTCGGTAGGTGGTCAGCTTACCGCCGAATACCGACAGCAGCGGTGCTTTGCCATTTTCATCATGAATATCAAGGGTGTAATCACGGGTAATAGCCTGCGGCGAGTCGGA > minE/2657999‑2658113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |