| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,659,376 | A→G | 18.4% | G35G (GGA→GGG) | glpE → | thiosulfate:cyanide sulfurtransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,659,376 | 0 | A | G | 18.4% | 106.2 / 13.7 | 70 | G35G (GGA→GGG) | glpE | thiosulfate:cyanide sulfurtransferase |
| Reads supporting (aligned to +/- strand): ref base A (25/32); new base G (7/6); total (32/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AAAGAGGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACTTT > minE/2659326‑2659442 | aaaGAGGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTc < 1:936934/69‑1 (MQ=255)aaaGAGGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTc < 1:330506/69‑1 (MQ=255)aaaGAGGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTc < 1:1860271/69‑1 (MQ=255)aaaGAGGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTc < 1:2868697/69‑1 (MQ=255) aaGAGGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTc < 1:1496149/68‑1 (MQ=255) aaGAGGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTc < 1:2535425/68‑1 (MQ=255) aaGAGGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTc < 1:2139971/68‑1 (MQ=255) gagGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTc < 1:1216822/66‑1 (MQ=255) ggCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCAttt < 1:58649/69‑1 (MQ=255) gCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCAttt < 1:99294/68‑1 (MQ=255) gTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAAcc < 1:3235994/69‑1 (MQ=255) gTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAAcc > 1:2357366/1‑63 (MQ=255) gTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGac > 1:1903726/1‑69 (MQ=255) gTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGac > 1:997161/1‑69 (MQ=255) gTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGac > 1:214220/1‑69 (MQ=255) gTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGac > 1:302850/1‑69 (MQ=255) gTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGac > 1:1090152/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCt > 1:1220219/1‑68 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:2067607/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:1227589/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:936053/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:726342/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:618030/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:1358864/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCTGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:1913820/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCt > 1:2498537/1‑68 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:2340517/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:2068146/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:524992/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:49012/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:41235/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:2897986/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:2300597/1‑69 (MQ=255) atTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTg > 1:1638336/1‑69 (MQ=255) cgcgATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTggg > 1:644892/1‑68 (MQ=255) cgcgATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGgc > 1:2785548/1‑69 (MQ=255) cgcgATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGgc > 1:987494/1‑69 (MQ=255) cgcgATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGgc > 1:728753/1‑69 (MQ=255) cgcgATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGgc > 1:546759/1‑69 (MQ=255) cgcgATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGgc > 1:458232/1‑69 (MQ=255) cgcgATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATATAACCAACGACACGCTGGgc > 1:502457/1‑69 (MQ=255) tCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCttt < 1:2743583/69‑1 (MQ=255) tCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCttt < 1:2229586/69‑1 (MQ=255) tCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCttt < 1:145777/69‑1 (MQ=255) cacaGAGTTTCGCAATGGGACTTGCGGTGCAGGCTTTCCAttt < 1:948728/43‑1 (MQ=255) caGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTAt > 1:1659580/1‑68 (MQ=255) caGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGAGCTTTTATg > 1:1889184/1‑69 (MQ=255) gTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTg < 1:3305477/69‑1 (MQ=255) gTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTg < 1:1390352/69‑1 (MQ=255) gTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTg < 1:1804686/69‑1 (MQ=255) gTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTg < 1:1471235/69‑1 (MQ=255) cGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCa < 1:2595721/38‑1 (MQ=255) cGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCa < 1:97085/38‑1 (MQ=255) cGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCa < 1:870151/38‑1 (MQ=255) cGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCa < 1:2592454/38‑1 (MQ=255) cGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCa < 1:1773169/38‑1 (MQ=255) cGCAATGGGGCATGCGGTGCAGGCTTTCCATTTAACCa < 1:3249597/38‑1 (MQ=255) tGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACt < 1:690239/69‑1 (MQ=255) tGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACt < 1:2785645/69‑1 (MQ=255) ggACATGCGTTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:345489/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:3210858/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:2432752/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:764716/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:820108/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:2133329/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:1311322/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:1585205/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:443683/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:2688351/69‑1 (MQ=255) ggACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACttt < 1:366963/69‑1 (MQ=255) | AAAGAGGCGGTGCTGGTCGATATTCGCGATCCACAGAGTTTCGCAATGGGACATGCGGTGCAGGCTTTCCATTTAACCAACGACACGCTGGGCGCTTTTATGCGTGATAACGACTTT > minE/2659326‑2659442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |