| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,662,258 | G→A | 100% | P191S (CCC→TCC) | rtcR ← | sigma 54‑dependent transcriptional regulator of rtcBA expression |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,662,258 | 0 | G | A | 79.1% | 21.0 / 1.1 | 19 | P191S (CCC→TCC) | rtcR | sigma 54‑dependent transcriptional regulator of rtcBA expression |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (15/0); total (17/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATCGGCGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGGGTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGCTG > minE/2662196‑2662325 | gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:2662199/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:934515/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:667320/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:532590/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:479576/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:343141/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:3415013/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:3307145/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:3038409/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:3009765/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:1014204/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:2651374/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:2315246/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:1595488/2‑69 (MQ=255)gaTCGGTGCGCGGGATTTGATCGCCACTTTTTAGATCTGCTCAATCATGCGGTTGAAGTGGGAGTTAcg > 1:2494273/2‑69 (MQ=255) tCGGCGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGGGTTAcgcg < 1:2604080/69‑1 (MQ=255) tCGGCGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGGGTTAcgcg < 1:3231954/69‑1 (MQ=255) cGGTTGAAGTGGGGGTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTc > 1:861620/1‑68 (MQ=255) cGGTTGAAGTGGGGGTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTc > 1:3112263/1‑68 (MQ=255) gggggTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:624735/1‑69 (MQ=255) gggggTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:1268875/1‑69 (MQ=255) gggggTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:1559472/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCgg > 1:1167339/1‑68 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:1138739/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:2392569/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:737210/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:648790/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:1556225/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:2112883/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:3018467/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:2135135/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:2955111/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:2231720/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:2439758/1‑69 (MQ=255) gggagTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:2403169/1‑69 (MQ=255) gggagTAACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGc > 1:439523/1‑69 (MQ=255) gggTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGCTg > 1:1715532/1‑69 (MQ=255) | AATCGGCGCGCGGGATTTGATCGCCACTTTTTCGATCTGCTCAATCATGCGGTTGAAGTGGGGGTTACGCGTGGCAATGCCGGACTTAAGAAAATCAAGCGTTTGCTGGCGTTCCTCGGCAAAGCGGCTG > minE/2662196‑2662325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |