| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,662,700 | T→C | 32.5% | R43R (CGA→CGG) | rtcR ← | sigma 54‑dependent transcriptional regulator of rtcBA expression |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,662,700 | 0 | T | C | 32.5% | 66.4 / 44.0 | 83 | R43R (CGA→CGG) | rtcR | sigma 54‑dependent transcriptional regulator of rtcBA expression |
| Reads supporting (aligned to +/- strand): ref base T (10/46); new base C (11/16); total (21/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
ATCGCGTTTAAGCGTTTCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCTG > minE/2662643‑2662765 | atCGCGTTTAAGCGTTTCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGa > 1:489741/1‑68 (MQ=255) ttAAGCGTTTCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGAt < 1:246244/69‑1 (MQ=255) ttAAGCGTTTCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGAt < 1:687130/69‑1 (MQ=255) ttAAGCGTTTCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGAt < 1:835668/69‑1 (MQ=255) tAAGCGTTTCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGAtt > 1:3151558/1‑69 (MQ=255) tAAGCGTTTCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGAt < 1:650300/68‑1 (MQ=255) tAAGCGTTTCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGAt < 1:3076120/68‑1 (MQ=255) tttCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGAt < 1:3342976/62‑1 (MQ=255) aTAGCGAGGGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:55602/44‑1 (MQ=37) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:1667511/44‑1 (MQ=38) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:2794569/44‑1 (MQ=38) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:1909686/44‑1 (MQ=38) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:1703929/44‑1 (MQ=38) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:1615559/44‑1 (MQ=38) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:1470944/44‑1 (MQ=38) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:1454524/44‑1 (MQ=38) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:3280043/44‑1 (MQ=38) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:346974/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:2322287/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:1163004/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:708057/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:2867891/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:2665142/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:2692919/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:1367879/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa < 1:42392/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa < 1:1797527/69‑1 (MQ=255) aTAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa < 1:1593613/69‑1 (MQ=255) tAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGccgatcg < 1:1161679/43‑1 (MQ=38) tAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:3046492/68‑1 (MQ=255) tAGCGAGCGCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:2989013/68‑1 (MQ=255) tAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa < 1:1561075/68‑1 (MQ=255) agcgagcgCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:1955225/67‑1 (MQ=255) agcgagcgCGAGCGGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:3343003/67‑1 (MQ=255) agcgagcgCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa > 1:6111/1‑67 (MQ=255) gcgagcgcgagcGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCATCGATTCTTGCTGGCATa > 1:970175/1‑66 (MQ=255) gcgagcgcgagcGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGAt < 1:2569264/53‑1 (MQ=255) gcgagcgcgagcGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa > 1:1378662/1‑66 (MQ=255) gcgagcgcgagcGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa > 1:1188771/1‑66 (MQ=255) gcgagcgcgagcGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa > 1:1290517/1‑66 (MQ=255) gcgagcgcgagcGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa > 1:1926787/1‑66 (MQ=255) gcgagcgcgagcGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa > 1:98355/1‑66 (MQ=255) gcgagcgcgagcGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATa > 1:774344/1‑66 (MQ=255) gagcgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:212182/69‑1 (MQ=255) gagcgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:2471419/69‑1 (MQ=255) gagcgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:2524031/69‑1 (MQ=255) gagcgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:116077/69‑1 (MQ=255) gagcgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:111227/69‑1 (MQ=255) gagcgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:2674322/69‑1 (MQ=255) gagcgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:1628432/69‑1 (MQ=255) cagcgcgtgcgGGCGTCGTGCAACTATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:313677/68‑1 (MQ=255) agcgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:3347099/68‑1 (MQ=255) gcgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:1507318/67‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCtt < 1:1431412/52‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCtt < 1:164516/52‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:2698397/69‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:984866/69‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:128661/69‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:3279480/69‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:825495/69‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:3010766/69‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:2082057/69‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:2106219/69‑1 (MQ=255) cgcgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:348728/69‑1 (MQ=255) cgcgagcgGGCGTCGCGCAACAATTCCAGTCGATCGATGACCAACGATTCCTGCTGGCATAAACAGAgt < 1:910220/69‑1 (MQ=255) cgagcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:1405917/67‑1 (MQ=255) agcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:3360311/65‑1 (MQ=255) gcgGGCGTCGTGCAATAATTCCGGCCGATCGATAAct > 1:3123315/1‑36 (MQ=19) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:2591250/1‑36 (MQ=25) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:684072/1‑36 (MQ=25) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:1361706/1‑36 (MQ=25) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:2794144/1‑36 (MQ=25) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:2768351/1‑36 (MQ=25) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:1364824/1‑36 (MQ=25) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:2398931/1‑36 (MQ=25) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:141048/1‑36 (MQ=25) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:2123060/1‑36 (MQ=25) gcgGGCGTCGTGCAATAATTCCAGCCGATCGATAAct > 1:1628723/1‑36 (MQ=25) gcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGAt < 1:938953/43‑1 (MQ=255) gcgGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACag < 1:1150052/61‑1 (MQ=255) cgGGCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:3172890/55‑1 (MQ=255) gggCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:2411379/54‑1 (MQ=255) ggCGTCGTGCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:1295052/53‑1 (MQ=255) gCAATAATTCCAGCCGATCGATAACTAACGATTCTTGCTGGCATa < 1:1603394/45‑1 (MQ=255) ccAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCt < 1:2127079/69‑1 (MQ=255) ccAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCt < 1:829567/69‑1 (MQ=255) ccAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCt < 1:689392/69‑1 (MQ=255) ccAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCt < 1:2128582/69‑1 (MQ=255) ccAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCt < 1:2565415/69‑1 (MQ=255) ccAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCt < 1:2838133/69‑1 (MQ=255) ccAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCt < 1:314475/69‑1 (MQ=255) aGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCTg < 1:376439/68‑1 (MQ=255) gTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAgt < 1:534375/41‑1 (MQ=255) | ATCGCGTTTAAGCGTTTCAAATAGCGAGCGCGAGCGGGCGTCGTGCAACAATTCCAGTCGATCGATGACCAACGATTCTTGCTGGCATAAACAGAGTGTCGGACGCCATTTTGACCAGCGCTG > minE/2662643‑2662765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |