| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,663,379 | T→C | 48.3% | G121G (GGT→GGC) | rtcB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,663,379 | 0 | T | C | 48.3% | ‑4.1 / 51.7 | 58 | G121G (GGT→GGC) | rtcB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (16/14); new base C (18/10); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GCGATTGAAACGGCCGTGCCGCACGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTT > minE/2663320‑2663447 | gCGATTGAAACGGCCGTGCCGCACGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGaa < 1:521158/69‑1 (MQ=255) cGTGCCGCACGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTaa < 1:1808062/69‑1 (MQ=255) cGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGaa > 1:1643785/1‑46 (MQ=255) cGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATg > 1:647203/1‑68 (MQ=255) tACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATg < 1:2307762/69‑1 (MQ=255) ggCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAAc < 1:2326053/48‑1 (MQ=255) ggCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAAc < 1:3242240/48‑1 (MQ=255) ggCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAAc < 1:3129318/48‑1 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:628142/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:2166315/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:2341915/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:2101999/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:205753/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:1031127/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:2521776/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:1975690/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:2567014/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:2965147/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:907828/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:693184/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:1474891/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:407693/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:471264/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:1234502/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga > 1:1329825/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGc > 1:2510630/1‑50 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag < 1:234685/69‑2 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag < 1:469046/69‑2 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag < 1:1897040/69‑2 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag < 1:1428075/69‑2 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag < 1:366303/69‑2 (MQ=255) tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTCAGCTGGag < 1:2112185/69‑2 (MQ=255) gTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag < 1:2299436/68‑2 (MQ=255) gTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag < 1:258261/68‑2 (MQ=255) tAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag < 1:1405006/67‑2 (MQ=255) aCGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag < 1:437817/64‑2 (MQ=255) aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc < 1:1047685/69‑1 (MQ=255) aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc < 1:721529/69‑1 (MQ=255) aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc < 1:122997/69‑1 (MQ=255) aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc < 1:1302375/69‑1 (MQ=255) aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc < 1:1864561/69‑1 (MQ=255) aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc < 1:2413736/69‑1 (MQ=255) aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc < 1:2548578/69‑1 (MQ=255) aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc < 1:1952252/69‑1 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATTGGCTGAGCTTGAAGCCGGTTATCAGt > 1:163944/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAg > 1:3061909/1‑68 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:2856675/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:451811/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:1408305/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:3244650/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:585856/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:1398951/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:2534914/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:66497/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:2040559/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:2044473/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:824290/1‑69 (MQ=255) aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt > 1:306580/1‑69 (MQ=255) tGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGtt < 1:3042300/69‑1 (MQ=255) tGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGtt < 1:45469/69‑1 (MQ=255) | GCGATTGAAACGGCCGTGCCGCACGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTT > minE/2663320‑2663447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |