Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,663,394 A→G 62.3% P126P (CCA→CCG rtcB → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,663,3940AG62.3% 47.2 / 50.8 82P126P (CCA→CCGrtcBconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (15/16);  new base G (26/25);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGCCGCACGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTTAACGCAAAA  >  minE/2663334‑2663456
                                                            |                                                              
cGTGCCGCACGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTaa                                                        <  1:1808062/69‑1 (MQ=255)
         cGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATg                                                >  1:647203/1‑68 (MQ=255)
               tACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATg                                         <  1:2307762/69‑1 (MQ=255)
                      ggCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAAc                                                       <  1:2326053/48‑1 (MQ=255)
                      ggCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAAc                                                       <  1:3129318/48‑1 (MQ=255)
                      ggCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAAc                                                       <  1:3242240/48‑1 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:2965147/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:2341915/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:2166315/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:2101999/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:205753/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:1031127/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:1975690/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:2521776/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:2567014/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:1474891/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:907828/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:693184/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:628142/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:407693/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:1234502/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:471264/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGcga                                             >  1:1329825/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGc                                               >  1:2510630/1‑50 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                            <  1:1897040/69‑2 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                            <  1:366303/69‑2 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                            <  1:469046/69‑2 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                            <  1:234685/69‑2 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                            <  1:1428075/69‑2 (MQ=255)
                            tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTCAGCTGGag                            <  1:2112185/69‑2 (MQ=255)
                             gTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                            <  1:258261/68‑2 (MQ=255)
                             gTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                            <  1:2299436/68‑2 (MQ=255)
                              tAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                            <  1:1405006/67‑2 (MQ=255)
                                 aCGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                            <  1:437817/64‑2 (MQ=255)
                                      aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                  <  1:2548578/69‑1 (MQ=255)
                                      aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                  <  1:2413736/69‑1 (MQ=255)
                                      aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                  <  1:721529/69‑1 (MQ=255)
                                      aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                  <  1:1047685/69‑1 (MQ=255)
                                      aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                  <  1:1952252/69‑1 (MQ=255)
                                      aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                  <  1:1864561/69‑1 (MQ=255)
                                      aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                  <  1:122997/69‑1 (MQ=255)
                                      aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                  <  1:1302375/69‑1 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATTGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:163944/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAg                >  1:3061909/1‑68 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:585856/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:3244650/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:1408305/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:1398951/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:306580/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:451811/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:2856675/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:66497/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:2534914/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:2040559/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:2044473/1‑69 (MQ=255)
                                         aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt               >  1:824290/1‑69 (MQ=255)
                                             tGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGtt           <  1:45469/69‑1 (MQ=255)
                                             tGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGtt           <  1:3042300/69‑1 (MQ=255)
                                             ggCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGtt           <  1:3192339/68‑1 (MQ=255)
                                                    gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                >  1:3178546/1‑57 (MQ=255)
                                                    gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                >  1:1079157/1‑57 (MQ=255)
                                                    gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                >  1:1464163/1‑57 (MQ=255)
                                                    gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                >  1:1044895/1‑57 (MQ=255)
                                                    gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                >  1:3207524/1‑57 (MQ=255)
                                                    gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                >  1:1490785/1‑57 (MQ=255)
                                                    gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                >  1:1471180/1‑57 (MQ=255)
                                                    gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                >  1:2598495/1‑57 (MQ=255)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:2810065/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:2196057/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:2344960/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:2099393/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:2556741/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:1638605/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:1127413/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:2765688/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:578161/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:2961647/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:1587369/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:41506/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:2315064/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:3434576/69‑1 (MQ=38)
                                                      aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCGGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa  <  1:1321797/69‑1 (MQ=38)
                                                            |                                                              
CGTGCCGCACGGGCGTACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTTAACGCAAAA  >  minE/2663334‑2663456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: