Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,663,412 T→G 52.3% A132A (GCT→GCG rtcB → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,663,4120TG52.3% 10.1 / 68.5 65A132A (GCT→GCGrtcBconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/13);  new base G (8/26);  total (26/39)
Fisher's exact test for biased strand distribution p-value = 5.82e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01

TACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTTAACGCAAAAATATCCCCGTTTCCTG  >  minE/2663349‑2663472
                                                               |                                                            
tACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATg                                                         <  1:2307762/69‑1 (MQ=255)
             tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                                            <  1:234685/69‑2 (MQ=255)
             tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                                            <  1:469046/69‑2 (MQ=255)
             tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                                            <  1:1428075/69‑2 (MQ=255)
             tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                                            <  1:366303/69‑2 (MQ=255)
             tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                                            <  1:1897040/69‑2 (MQ=255)
             tGTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTCAGCTGGag                                            <  1:2112185/69‑2 (MQ=255)
              gTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                                            <  1:2299436/68‑2 (MQ=255)
              gTAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                                            <  1:258261/68‑2 (MQ=255)
               tAAACGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                                            <  1:1405006/67‑2 (MQ=255)
                  aCGTGATAAAGGCGCATGGGAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGag                                            <  1:437817/64‑2 (MQ=255)
                       aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                                  <  1:1047685/69‑1 (MQ=255)
                       aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                                  <  1:2413736/69‑1 (MQ=255)
                       aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                                  <  1:2548578/69‑1 (MQ=255)
                       aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                                  <  1:1952252/69‑1 (MQ=255)
                       aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                                  <  1:1864561/69‑1 (MQ=255)
                       aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                                  <  1:1302375/69‑1 (MQ=255)
                       aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                                  <  1:122997/69‑1 (MQ=255)
                       aTAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATc                                  <  1:721529/69‑1 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATTGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:163944/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAg                                >  1:3061909/1‑68 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:2856675/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:306580/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:3244650/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:451811/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:585856/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:2534914/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:66497/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:824290/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:1398951/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:2044473/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:2040559/1‑69 (MQ=255)
                          aaGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGt                               >  1:1408305/1‑69 (MQ=255)
                              tGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGtt                           <  1:45469/69‑1 (MQ=255)
                              tGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGtt                           <  1:3042300/69‑1 (MQ=255)
                              ggCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGtt                           <  1:3192339/68‑1 (MQ=255)
                                     gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                                >  1:3178546/1‑57 (MQ=255)
                                     gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                                >  1:1044895/1‑57 (MQ=255)
                                     gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                                >  1:1079157/1‑57 (MQ=255)
                                     gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                                >  1:1464163/1‑57 (MQ=255)
                                     gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                                >  1:1471180/1‑57 (MQ=255)
                                     gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                                >  1:1490785/1‑57 (MQ=255)
                                     gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                                >  1:3207524/1‑57 (MQ=255)
                                     gAAAATCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAg                                >  1:2598495/1‑57 (MQ=255)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:41506/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:2315064/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:1127413/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:578161/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:2344960/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:2099393/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:2556741/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:2961647/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:3434576/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:2765688/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:2810065/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:2196057/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:1587369/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:1638605/69‑1 (MQ=38)
                                       aaaTCCGCCTGTTAACGTCGATGCGAAATGGGCGGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:1321797/69‑1 (MQ=38)
                                               cTGTTAACGTCGATGCGAAATGGGCTGAGCTGGAGGCCGGTTATCAGTGGTTAACGCaaaa                  <  1:234327/61‑1 (MQ=38)
                                                    aaCGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTTAACGCAAAAATATCCCCGttt      >  1:503205/1‑68 (MQ=255)
                                                    aaCGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTTAACGCAAAAATATCCCCGTTTc     >  1:1711877/1‑69 (MQ=255)
                                                    aaCGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTTAACGCAAAAATATCCCCGTTTc     >  1:1972469/1‑69 (MQ=255)
                                                    aaCGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTTAACGCAAAAATATCCCCGTTTc     >  1:1688997/1‑69 (MQ=255)
                                                       gTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTTAACGCAAAAATATCCCCGTTTCCTg  <  1:3374436/69‑1 (MQ=255)
                                                               |                                                            
TACCACTGGCCGTTGTAAACGTGATAAAGGTGCCTGGGAAAATCCACCTGTTAACGTCGATGCTAAATGGGCTGAGCTTGAAGCCGGTTATCAGTGGTTAACGCAAAAATATCCCCGTTTCCTG  >  minE/2663349‑2663472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: