| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,664,111 | A→G | 26.8% | E365E (GAA→GAG) | rtcB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,664,111 | 0 | A | G | 26.8% | 144.0 / 54.3 | 130 | E365E (GAA→GAG) | rtcB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (26/69); new base G (9/26); total (35/95) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
AGCCGAACTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGA > minE/2664046‑2664175 | gCCGAACTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGc < 1:2269985/68‑1 (MQ=255) aCTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTaaa < 1:849353/69‑1 (MQ=255) aCTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTaaa < 1:2167755/69‑1 (MQ=255) aCTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTaaa < 1:2168311/69‑1 (MQ=255) aCTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTaaa < 1:1470777/69‑1 (MQ=255) aCTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTaaa < 1:3435184/69‑1 (MQ=255) aCTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTaaa < 1:1092908/69‑1 (MQ=255) aCTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTaaa < 1:1718572/69‑1 (MQ=255) aCTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCAACGCGCATGTGGAATGCCGTaaa < 1:604991/69‑1 (MQ=255) tAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTaaa < 1:1055688/67‑1 (MQ=255) aaaGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGa > 1:2368970/1‑68 (MQ=255) aaaGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGAt > 1:2359818/1‑69 (MQ=255) aGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGACACCGCGCATGTGGAATGCCGTAAAGa > 1:3044764/1‑66 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCTCCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:3324780/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:57112/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:1862759/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2092924/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:62608/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:611429/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:248441/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2526938/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2596575/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:269418/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2752991/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2772285/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:915278/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:744524/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:83337/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:1441018/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:1252901/69‑1 (MQ=255) aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:1518255/69‑1 (MQ=255) aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg < 1:1742167/69‑1 (MQ=255) aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg < 1:1094873/69‑1 (MQ=255) aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg < 1:3223149/69‑1 (MQ=255) aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg < 1:2137873/69‑1 (MQ=255) aaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:3088167/68‑1 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:892558/69‑1 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:3183916/1‑69 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:1105733/1‑69 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:287603/1‑69 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:818359/1‑69 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:3164908/1‑60 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:1340188/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:3176446/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:118923/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:2683461/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:1817821/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:1502619/1‑60 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:1581405/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:1780043/60‑1 (MQ=255) aTCAAATTCGTGCCACCGCGCATGTGGAATGCCGTaaa < 1:487621/38‑1 (MQ=255) cAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCg > 1:1469682/1‑69 (MQ=255) cGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATaaa < 1:1907338/69‑1 (MQ=255) cGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta > 1:1993731/1‑65 (MQ=255) cGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta > 1:2472249/1‑65 (MQ=255) cGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta > 1:614224/1‑65 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:434505/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:2006088/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:1516802/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:776836/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:2701969/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:1073364/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGat > 1:1046120/1‑67 (MQ=255) aCCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATAtt < 1:2122163/69‑1 (MQ=255) tgtgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:1634910/69‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1008592/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:51863/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:706687/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:2501553/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:3131348/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1307459/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1212417/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1201055/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:3158040/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:2485190/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:2458406/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:1290134/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:186636/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:2408913/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:3377167/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:2230773/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:196277/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:1924665/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:1921600/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt > 1:990225/1‑67 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:3231815/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt > 1:3224424/1‑67 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:3130450/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:2562760/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:295797/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:2717046/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:1920533/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:630786/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:2508357/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:874947/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:2766817/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATACCGATGGCGTATAAAGATATTGATGCGGt > 1:962891/1‑67 (MQ=255) tgGAATGCCGTAAAGATGACGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt > 1:2437604/1‑67 (MQ=255) ggAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1665573/39‑1 (MQ=255) ggAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:1875524/66‑1 (MQ=255) | AGCCGAACTAAAGCGAAAAAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGA > minE/2664046‑2664175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |