| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,664,129 | A→G | 29.4% | E371E (GAA→GAG) | rtcB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,664,129 | 0 | A | G | 29.4% | 119.1 / 57.2 | 122 | E371E (GAA→GAG) | rtcB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (23/63); new base G (9/27); total (32/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
AAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGA > minE/2664064‑2664191 | aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCTCCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:3324780/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:1843373/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2526938/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2596575/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2631344/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:248441/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2752991/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2092924/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:1862759/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:57112/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:1518255/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:62608/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:915278/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:1085342/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:744524/69‑1 (MQ=255)aaaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:83337/69‑1 (MQ=255)aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg < 1:3223149/69‑1 (MQ=255)aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg < 1:2137873/69‑1 (MQ=255)aaaCTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTg < 1:835602/69‑1 (MQ=255) aaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:3088167/68‑1 (MQ=255) aaCTGTTCAGTGTGGAAGATCAAATCCGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTg < 1:2154657/68‑1 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:818359/1‑69 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:1730956/1‑69 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:287603/1‑69 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:1105733/1‑69 (MQ=255) cAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:3183916/1‑69 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:1340188/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:2416677/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:3176446/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:1502619/1‑60 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:118923/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:3289574/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:2683461/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga > 1:897552/1‑60 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:1424470/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:1817821/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:1780043/60‑1 (MQ=255) aGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATcgacga < 1:1581405/60‑1 (MQ=255) cAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCg > 1:1469682/1‑69 (MQ=255) cGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATaaa < 1:3206180/69‑1 (MQ=255) cGTGCCACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATaaa < 1:1907338/69‑1 (MQ=255) cGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta > 1:614224/1‑65 (MQ=255) cGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta > 1:2472249/1‑65 (MQ=255) cGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta > 1:1993731/1‑65 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:2006088/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:2701969/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:1516802/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:434505/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:1073364/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGata > 1:776836/1‑68 (MQ=255) cACCGCGCATGTGGAGTGCCGTAAAGATGCCGAGGTGATCGACGAAATCCCGATGGCGTATAAAGat > 1:1046120/1‑67 (MQ=255) tgtgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:1634910/69‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1201055/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1225016/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:3131348/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1307459/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1212417/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:51863/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:74198/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1008592/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:614934/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:2501553/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:706687/40‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:583005/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:3158040/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:186636/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:3377167/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:1472840/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:1290134/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:1921600/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:1924665/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:196277/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:2230773/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:2408913/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:2458406/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGta < 1:2485190/49‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:3231815/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:3134205/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:3130450/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt > 1:2306040/1‑67 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:295797/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:2717046/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt > 1:990225/1‑67 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt < 1:2562760/67‑1 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:2508357/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:874947/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:630786/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:1920533/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGa > 1:2766817/1‑69 (MQ=255) tgGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATACCGATGGCGTATAAAGATATTGATGCGGt > 1:962891/1‑67 (MQ=255) tgGAATGCCGTAAAGATGCCAAAGTGATCGACGAAATCCCGATGGCGta < 1:2702174/49‑1 (MQ=255) tgGAATGCCGTAAAGATGACGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGt > 1:2437604/1‑67 (MQ=255) ggAATGCCGTAAAGATGCCGAAGTGATCGACGAAATccc < 1:1665573/39‑1 (MQ=255) aaGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGa < 1:733096/43‑1 (MQ=255) aaGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGa < 1:1057967/43‑1 (MQ=255) gATGCCGAGGTGATCGACGAAATCCCGATGGCGTATaaa < 1:2890656/39‑1 (MQ=255) tGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGa < 1:719153/69‑1 (MQ=255) tGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGa < 1:3306082/69‑1 (MQ=255) tGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGa < 1:1996339/69‑1 (MQ=255) tGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGa < 1:2936719/69‑1 (MQ=255) | AAACTGTTCAGCGTGGAAGATCAAATTCGTGCCACCGCGCATGTGGAATGCCGTAAAGATGCCGAAGTGATCGACGAAATCCCGATGGCGTATAAAGATATTGATGCGGTGATGGCGGCACAAAGCGA > minE/2664064‑2664191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |