| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 227,540 | A→G | 18.7% | T112A (ACG→GCG) | crl → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 227,540 | 0 | A | G | 18.7% | 75.1 / 9.9 | 53 | T112A (ACG→GCG) | crl | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (38/5); new base G (3/7); total (41/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.54e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01 | |||||||||||
CACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGC > minE/227473‑227604 | cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:2705043/1‑69 (MQ=255)cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:2958733/1‑69 (MQ=255)cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:3035679/1‑69 (MQ=255)cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:2059207/1‑69 (MQ=255)cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:3420277/1‑69 (MQ=255)cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:455622/1‑69 (MQ=255)cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:1619916/1‑69 (MQ=255)cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:116082/1‑69 (MQ=255)cacTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgac > 1:1334050/1‑69 (MQ=255) gAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGacgacg > 1:665101/1‑69 (MQ=255) gAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGacgacg > 1:2585842/1‑69 (MQ=255) ggTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGaa > 1:2990706/1‑69 (MQ=255) ggTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGaa > 1:2375904/1‑69 (MQ=255) ggTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGa > 1:3228813/1‑68 (MQ=255) ggTTGAACGACTGGAGCACACCATGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGaa > 1:584141/1‑69 (MQ=255) gTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAAt > 1:1690027/1‑69 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTTCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:83764/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:2715066/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:924377/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:329984/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:596310/69‑1 (MQ=255) cGATTGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:2940824/69‑1 (MQ=255) gattGGAGCACACCCTGCGTGAATTTCACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAg < 1:3125062/65‑1 (MQ=255) cacCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACcgg > 1:3325121/1‑68 (MQ=255) cacCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACcgg > 1:210194/1‑68 (MQ=255) cacCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACcgg > 1:1002125/1‑68 (MQ=255) cacCCTACGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACcgg > 1:5030/1‑68 (MQ=255) gCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGATTCTGAAGCTGGAACCGGCGgatga > 1:1552064/1‑69 (MQ=255) gCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGgatga > 1:1104842/1‑69 (MQ=255) gCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGgatga > 1:2500118/1‑69 (MQ=255) gCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGgatga > 1:2785851/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGaa > 1:3387593/1‑51 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:659103/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:716738/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:2581773/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:2465730/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:1760950/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:2402411/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:1607577/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:1587135/1‑69 (MQ=255) cACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAGCt > 1:1873687/1‑44 (MQ=38) cACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAGCTGGAACCGGCAGATGATTTTCGTGa > 1:1628862/1‑68 (MQ=255) cACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAGCTGGAACCGGCAGATGATTTTCGTGa > 1:3347633/1‑68 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:2056010/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:164187/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:512026/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:2166785/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:3123686/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:1467696/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:141730/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:2646620/1‑67 (MQ=255) gCGTGAACTGCTGACGACGCTGAATGTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGaa > 1:2398965/1‑69 (MQ=255) gCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGaa > 1:1857766/1‑69 (MQ=255) tGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:1470458/69‑1 (MQ=255) tGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:401956/69‑1 (MQ=255) tGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:2828128/69‑1 (MQ=255) tGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGACGATTTTCGTGACGAGCCGGTGAAGtt < 1:1868453/69‑1 (MQ=255) gAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:2580942/68‑1 (MQ=255) ctgctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACg > 1:2427149/1‑69 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:1691162/1‑68 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:347347/1‑68 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:2331780/1‑68 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:2919848/1‑68 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:985672/1‑68 (MQ=255) acgacgCTGAATCTGCAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:2023072/59‑1 (MQ=255) | CACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGC > minE/227473‑227604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |