| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,664,355 | A→G | 100% | S37G (AGC→GGC) | rtcA → | RNA 3'‑terminal phosphate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,664,355 | 0 | A | G | 80.1% | 24.2 / 1.1 | 20 | S37G (AGC→GGC) | rtcA | RNA 3'‑terminal phosphate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/4); new base G (16/0); total (16/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGATCCTGCGCTCGGCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCAGCATTCGTGCCGGGCGGGCGAAACCGGGGCTGTTGCGCCAGCATCTG > minE/2664296‑2664402 | aGATCCTGCGCTCGGCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCAt > 1:1403948/1‑64 (MQ=255)aGATCCTGCGCTCGGCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCAt > 1:14055/1‑64 (MQ=255)aGATCCTGCGCTCGGCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCAt > 1:1512875/1‑64 (MQ=255)aGATCCTGCGCTCGGCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCAt > 1:166541/1‑64 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCa > 1:2285659/1‑39 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTg > 1:1230614/1‑45 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCgggg > 1:1992462/1‑65 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCGGGGc > 1:1320667/1‑66 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCGGGGc > 1:415052/1‑66 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCGGGGc > 1:344147/1‑66 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCGGGGc > 1:2844531/1‑66 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCGGGGc > 1:2701348/1‑66 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCGGGGc > 1:2501493/1‑66 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCGGGGc > 1:2481334/1‑66 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCGGGGc > 1:2232406/1‑66 (MQ=255) tGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCCGGGCGGGCGAAACCGGGGc > 1:138813/1‑66 (MQ=255) ggCCAGCCATTTACCATCACCAGCATTCGTGCCGGGCGGGCGAAACCGGGGCTGTTGCGCCAGCATCTg < 1:1452206/69‑1 (MQ=255) ggCCAGCCATTTACCATCACCAGCATTCGTGCCGGGCGGGCGAAACCGGGGCTGTTGCGCCAGCATCTg < 1:657785/69‑1 (MQ=255) gccagccaTTTACCATCACCAGCATTCGTGCCGGGCGGGCGAAACCGGGGCTGTTGCGCCAGCATCTg < 1:1349906/68‑1 (MQ=255) gccagccaTTTACCATCACCAGCATTCGTGCCGGGCGGGCGAAACCGGGGCTGTTGCGCCAGCATCTg < 1:876613/68‑1 (MQ=255) | AGATCCTGCGCTCGGCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCAGCATTCGTGCCGGGCGGGCGAAACCGGGGCTGTTGCGCCAGCATCTG > minE/2664296‑2664402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |