| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,665,357 | A→G | 24.9% | D885D (GAT→GAC) ‡ | malT ← | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,665,357 | 0 | A | G | 24.9% | 128.3 / 39.4 | 108 | D885D (GAT→GAC) ‡ | malT | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Reads supporting (aligned to +/- strand): ref base A (65/16); new base G (3/24); total (68/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
ATCCGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCC > minE/2665294‑2665423 | aTCCGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTg > 1:1732637/1‑69 (MQ=255) cGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGc < 1:2129656/68‑1 (MQ=255) cGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGc < 1:2782936/68‑1 (MQ=255) cGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGc < 1:1649391/68‑1 (MQ=255) cGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGc < 1:1024902/68‑1 (MQ=255) cGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGc < 1:1144880/68‑1 (MQ=255) cGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGc < 1:570839/68‑1 (MQ=255) cGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGc < 1:725851/68‑1 (MQ=255) cGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGc < 1:890079/68‑1 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:1945723/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:2707552/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:1791215/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:2379452/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:194468/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:1704605/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:1506022/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:1932438/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:1048767/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:762517/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:491976/68‑2 (MQ=255) gCTAAACTCATACCCCATATCCCATCATCTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACgg < 1:1294302/68‑2 (MQ=255) ccccATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCt > 1:1842754/1‑68 (MQ=255) ccccATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCt > 1:2871536/1‑68 (MQ=255) ccccATCATCTTCAGCAATTGCTGGGCGTGTTGTACAGCATCCTGGCGATGGGCCACGCCGAGTTTCt > 1:1445987/1‑68 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1319307/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:443434/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1797706/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1269250/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:2948262/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1452124/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:2811563/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1600158/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1554729/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1545065/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:2460325/68‑1 (MQ=255) tcatcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACa < 1:2526082/69‑1 (MQ=255) tcatcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACa < 1:2232645/69‑1 (MQ=255) tcatcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACa < 1:1878680/69‑1 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTTGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:784675/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1446456/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1126223/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:2795603/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:2412783/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:358732/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1096322/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:3376257/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1769356/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1816850/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:3354110/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1915510/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCCCGCCGAGTTTCTGATACAGAtt > 1:3065816/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:2075353/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:3324128/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:2267119/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:1082973/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:1865261/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:922779/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:2520122/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:2525510/1‑69 (MQ=255) cAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGa < 1:1972457/68‑1 (MQ=255) tGCTGGGCGTGTTTTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:1540702/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATTCAGATTGCGGATATGc > 1:816707/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:1760018/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:2246659/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:2949824/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:2032703/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:3200593/1‑69 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGGCACGCCGAGTTTCTGATACAGATTGCg > 1:2688065/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:640852/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1134525/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:672781/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1336694/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1258624/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1259403/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:801460/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1886445/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:2859115/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1377208/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1458633/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCg > 1:1317318/1‑69 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCg > 1:2780871/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:2655076/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:1833354/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:887447/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:1955532/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:2307154/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:3314104/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:525951/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:3416688/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:3027345/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:94673/1‑69 (MQ=255) ttgtACCGAATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:726427/1‑69 (MQ=255) gtACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTACAGATTGCGGATATGCGTCTTGAtggtgg > 1:2157443/1‑69 (MQ=255) gtACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTACAGATTGCGGATATGCGTCTTGAtggtgg > 1:2053725/1‑69 (MQ=255) gtACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTACAGATTGCGGATATGCGTCTTGAtggtgg > 1:932243/1‑69 (MQ=255) gtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggtg < 1:1497569/68‑1 (MQ=255) gCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATTCGTTTTGATGGTGGTTGcc > 1:709264/1‑69 (MQ=255) gCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGcc > 1:88386/1‑69 (MQ=255) gCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGcc > 1:3060153/1‑69 (MQ=255) cATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGcc > 1:1924159/1‑68 (MQ=255) | ATCCGGCTAAACTTACACGCCGTACCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCC > minE/2665294‑2665423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |