| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,665,375 | G→A | 14.8% | G879G (GGC→GGT) | malT ← | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,665,375 | 0 | G | A | 14.8% | 163.7 / 13.5 | 93 | G879G (GGC→GGT) | malT | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (69/10); new base A (3/11); total (72/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCCGCGACTTCCAGTTCTC > minE/2665318‑2665439 | ccccATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCt > 1:2871536/1‑68 (MQ=255)ccccATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCt > 1:1842754/1‑68 (MQ=255)ccccATCATCTTCAGCAATTGCTGGGCGTGTTGTACAGCATCCTGGCGATGGGCCACGCCGAGTTTCt > 1:1445987/1‑68 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1545065/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1554729/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1452124/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1600158/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:2811563/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1319307/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1797706/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:1269250/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:443434/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:2460325/68‑1 (MQ=255) atcatcTTCAGCAGTTTCTGCGCGTGTTGTACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTa < 1:2948262/68‑1 (MQ=255) tcatcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACa < 1:1878680/69‑1 (MQ=255) tcatcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACa < 1:2232645/69‑1 (MQ=255) tcatcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACa < 1:2526082/69‑1 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTTGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:784675/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1769356/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1816850/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1446456/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:2795603/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1915510/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:3354110/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:3376257/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1321206/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:358732/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1126223/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:1096322/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:2412783/1‑69 (MQ=255) atcTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGa > 1:981986/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCCCGCCGAGTTTCTGATACAGAtt > 1:3065816/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:1865261/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:3324128/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:2525510/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:2520122/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:2075353/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:922779/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:1082973/1‑69 (MQ=255) cTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGAtt > 1:2267119/1‑69 (MQ=255) cAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGa < 1:2751506/68‑1 (MQ=255) cAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGa < 1:1972457/68‑1 (MQ=255) cAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGa < 1:2159399/68‑1 (MQ=255) tGCTGGGCGTGTTTTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:1540702/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATTCAGATTGCGGATATGc > 1:816707/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:2949824/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:2246659/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:3200593/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:2032703/1‑69 (MQ=255) tGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGc > 1:1760018/1‑69 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGGCACGCCGAGTTTCTGATACAGATTGCg > 1:2688065/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1134525/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1458633/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1377208/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1336694/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1259403/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:2859115/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1886445/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:640852/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:672781/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:1258624/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCg > 1:801460/1‑61 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCg > 1:2780871/1‑69 (MQ=255) gCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCg > 1:1317318/1‑69 (MQ=255) cGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGtttt < 1:1896313/67‑1 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:525951/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:3416688/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:887447/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:3314104/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:2307154/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:1631487/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:3027345/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:1955532/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:1833354/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:94673/1‑69 (MQ=255) ttgtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:2655076/1‑69 (MQ=255) ttgtACCGAATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggt > 1:726427/1‑69 (MQ=255) gtACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTACAGATTGCGGATATGCGTCTTGAtggtgg > 1:2157443/1‑69 (MQ=255) gtACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTACAGATTGCGGATATGCGTCTTGAtggtgg > 1:932243/1‑69 (MQ=255) gtACCGCGGCCTGACGGTGGGCGACACCGAGTTTTTGGTACAGATTGCGGATATGCGTCTTGAtggtgg > 1:2053725/1‑69 (MQ=255) gtACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGAtggtg < 1:1497569/68‑1 (MQ=255) gCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATTCGTTTTGATGGTGGTTGcc > 1:709264/1‑69 (MQ=255) gCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATTCGTTTTGATGGTGGTTGcc > 1:713171/1‑69 (MQ=255) gCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGcc > 1:3060153/1‑69 (MQ=255) gCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGcc > 1:88386/1‑69 (MQ=255) cATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGcc > 1:1924159/1‑68 (MQ=255) tCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCcgcg < 1:435308/69‑1 (MQ=255) tCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCcgcg < 1:3187749/69‑1 (MQ=255) gCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCCGCGACt > 1:3355075/1‑67 (MQ=255) gggCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCCGCGACTTCCAGtt > 1:1026229/1‑69 (MQ=255) gggCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCCGCGACTTCCAGtt > 1:1845902/1‑69 (MQ=255) ccACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCCGCGACTTCCAGTtctc > 1:1152250/1‑69 (MQ=255) ccACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCCGCGACTTCCAGTtctc > 1:1072549/1‑69 (MQ=255) | CCCCATCATCTTCAGCAATTGCTGGGCGTGTTGTACCGCATCCTGGCGATGGGCCACGCCGAGTTTCTGATACAGATTGCGGATATGCGTTTTGATGGTGGTTGCCGCGACTTCCAGTTCTC > minE/2665318‑2665439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |