| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,665,537 | A→C | 31.2% | P825P (CCT→CCG) | malT ← | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,665,537 | 0 | A | C | 31.2% | 71.5 / 54.4 | 77 | P825P (CCT→CCG) | malT | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Reads supporting (aligned to +/- strand): ref base A (33/20); new base C (20/4); total (53/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01 | |||||||||||
CAGCCCCAGTACCTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAATTTATGCCGAT > minE/2665474‑2665595 | cAGCCCCAGTACCTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGgatg > 1:1476794/1‑69 (MQ=255) ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc > 1:2582165/1‑69 (MQ=255) ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc > 1:2569026/1‑69 (MQ=255) ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc > 1:2546874/1‑69 (MQ=255) ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc > 1:1422435/1‑69 (MQ=255) ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc > 1:1453070/1‑69 (MQ=255) ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc > 1:2242552/1‑69 (MQ=255) aTTCACGTTGGGTCAACGGGCTTGTGCGGATCAGCTCTGGCACTTCCGGATGATTTAGCAGACGTTCa < 1:2210125/68‑1 (MQ=255) aTTCACGTTGGGTCAACGGGCTGGTGCGGATCAGCTCTGGCACTTCCGGATGATTTAGCAGTCGTTCa < 1:439100/68‑1 (MQ=255) aTTCACGTTGGGTCAACGGGCTGGTGCGGATCAGCTCTGGCACTTCCGGATGATTTAGCAGACGTTCa < 1:600393/68‑1 (MQ=255) aTTCACGTTGGGTCAACGGGCTGGTGCGGATCAGCTCTGGCACTTCCGGATGATTTAGCAGACGTTCa < 1:1787268/68‑1 (MQ=255) tCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAAc > 1:2040293/1‑68 (MQ=255) tCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAAc > 1:2774897/1‑68 (MQ=255) tCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAAc > 1:1739382/1‑68 (MQ=255) cACGTTGCGTCAGCGGTCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:3012800/1‑64 (MQ=255) cACGTTGCGTCAGCGGGGTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:1099065/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:376884/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:354658/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:3325730/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:3093383/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:2868145/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:2784750/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:2152551/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:246370/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:2678400/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:388217/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:1281335/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:2034891/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:1535914/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:928500/1‑64 (MQ=255) cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:87570/1‑64 (MQ=255) cACGTTACGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:701780/1‑64 (MQ=255) cACGCTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc > 1:63726/1‑64 (MQ=255) gCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATtct < 1:1457968/69‑1 (MQ=255) aGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATc > 1:2196685/1‑68 (MQ=255) aGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATc > 1:2049676/1‑68 (MQ=255) aGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATc > 1:1610677/1‑68 (MQ=255) aGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATc > 1:1432907/1‑68 (MQ=255) cGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa < 1:2984039/68‑1 (MQ=255) cGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa < 1:2573716/68‑1 (MQ=255) cGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa < 1:2129343/68‑1 (MQ=255) gCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa < 1:1653423/65‑1 (MQ=255) cTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa < 1:265173/64‑1 (MQ=255) gCGGATCAGTTCAGGTACTTCAGTATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCg < 1:2763687/67‑1 (MQ=255) gCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCg < 1:1327237/67‑1 (MQ=255) gCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCg < 1:2510421/67‑1 (MQ=255) gCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCg < 1:131581/67‑1 (MQ=255) gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt < 1:2387023/68‑1 (MQ=255) gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt < 1:744354/68‑1 (MQ=255) gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt < 1:526231/68‑1 (MQ=255) gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt < 1:2457096/68‑1 (MQ=255) gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt < 1:1379693/68‑1 (MQ=255) gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt < 1:2380834/68‑1 (MQ=255) gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt < 1:143505/68‑1 (MQ=255) gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt < 1:2045883/68‑1 (MQ=255) cAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAATTTATGCCgat < 1:2270767/69‑1 (MQ=255) tACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt < 1:582301/56‑1 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:2061842/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:1318034/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:912184/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:1433046/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:1551007/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:1868024/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:1929185/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:420651/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:413430/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:2492838/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:2306141/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:2362495/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:3225632/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:3164795/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:2375580/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:238415/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:1013537/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:2827763/1‑40 (MQ=255) ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg > 1:2431882/1‑40 (MQ=255) ttCCGGATGATTAAGCAGACGTTCAACGAAATTCTCATCg > 1:990500/1‑40 (MQ=38) | CAGCCCCAGTACCTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAATTTATGCCGAT > minE/2665474‑2665595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |