Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,665,537 A→C 31.2% P825P (CCT→CCG malT ← DNA‑binding transcriptional activator, maltotriose‑ATP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,665,5370AC31.2% 71.5 / 54.4 77P825P (CCT→CCGmalTDNA‑binding transcriptional activator, maltotriose‑ATP‑binding
Reads supporting (aligned to +/- strand):  ref base A (33/20);  new base C (20/4);  total (53/24)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

CAGCCCCAGTACCTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAATTTATGCCGAT  >  minE/2665474‑2665595
                                                               |                                                          
cAGCCCCAGTACCTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGgatg                                                       >  1:1476794/1‑69 (MQ=255)
           ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc                                            >  1:2582165/1‑69 (MQ=255)
           ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc                                            >  1:2569026/1‑69 (MQ=255)
           ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc                                            >  1:2546874/1‑69 (MQ=255)
           ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc                                            >  1:1422435/1‑69 (MQ=255)
           ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc                                            >  1:1453070/1‑69 (MQ=255)
           ccTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGAc                                            >  1:2242552/1‑69 (MQ=255)
                 aTTCACGTTGGGTCAACGGGCTTGTGCGGATCAGCTCTGGCACTTCCGGATGATTTAGCAGACGTTCa                                       <  1:2210125/68‑1 (MQ=255)
                 aTTCACGTTGGGTCAACGGGCTGGTGCGGATCAGCTCTGGCACTTCCGGATGATTTAGCAGTCGTTCa                                       <  1:439100/68‑1 (MQ=255)
                 aTTCACGTTGGGTCAACGGGCTGGTGCGGATCAGCTCTGGCACTTCCGGATGATTTAGCAGACGTTCa                                       <  1:600393/68‑1 (MQ=255)
                 aTTCACGTTGGGTCAACGGGCTGGTGCGGATCAGCTCTGGCACTTCCGGATGATTTAGCAGACGTTCa                                       <  1:1787268/68‑1 (MQ=255)
                   tCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAAc                                     >  1:2040293/1‑68 (MQ=255)
                   tCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAAc                                     >  1:2774897/1‑68 (MQ=255)
                   tCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAAc                                     >  1:1739382/1‑68 (MQ=255)
                    cACGTTGCGTCAGCGGTCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:3012800/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGGTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:1099065/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:376884/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:354658/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:3325730/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:3093383/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:2868145/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:2784750/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:2152551/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:246370/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:2678400/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:388217/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:1281335/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:2034891/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:1535914/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:928500/1‑64 (MQ=255)
                    cACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:87570/1‑64 (MQ=255)
                    cACGTTACGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:701780/1‑64 (MQ=255)
                    cACGCTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTc                                        >  1:63726/1‑64 (MQ=255)
                          gCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATtct                             <  1:1457968/69‑1 (MQ=255)
                               aGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATc                         >  1:2196685/1‑68 (MQ=255)
                               aGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATc                         >  1:2049676/1‑68 (MQ=255)
                               aGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATc                         >  1:1610677/1‑68 (MQ=255)
                               aGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATc                         >  1:1432907/1‑68 (MQ=255)
                                 cGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa                       <  1:2984039/68‑1 (MQ=255)
                                 cGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa                       <  1:2573716/68‑1 (MQ=255)
                                 cGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa                       <  1:2129343/68‑1 (MQ=255)
                                    gCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa                       <  1:1653423/65‑1 (MQ=255)
                                     cTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGa                       <  1:265173/64‑1 (MQ=255)
                                          gCGGATCAGTTCAGGTACTTCAGTATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCg               <  1:2763687/67‑1 (MQ=255)
                                          gCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCg               <  1:1327237/67‑1 (MQ=255)
                                          gCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCg               <  1:2510421/67‑1 (MQ=255)
                                          gCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCg               <  1:131581/67‑1 (MQ=255)
                                             gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt           <  1:2387023/68‑1 (MQ=255)
                                             gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt           <  1:744354/68‑1 (MQ=255)
                                             gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt           <  1:526231/68‑1 (MQ=255)
                                             gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt           <  1:2457096/68‑1 (MQ=255)
                                             gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt           <  1:1379693/68‑1 (MQ=255)
                                             gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt           <  1:2380834/68‑1 (MQ=255)
                                             gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt           <  1:143505/68‑1 (MQ=255)
                                             gATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt           <  1:2045883/68‑1 (MQ=255)
                                                     cAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAATTTATGCCgat  <  1:2270767/69‑1 (MQ=255)
                                                         tACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAAtt           <  1:582301/56‑1 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:2061842/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:1318034/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:912184/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:1433046/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:1551007/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:1868024/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:1929185/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:420651/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:413430/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:2492838/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:2306141/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:2362495/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:3225632/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:3164795/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:2375580/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:238415/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:1013537/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:2827763/1‑40 (MQ=255)
                                                            ttCCGGATGATTTAGCAGACGTTCAACGAAATTCTCATCg                        >  1:2431882/1‑40 (MQ=255)
                                                            ttCCGGATGATTAAGCAGACGTTCAACGAAATTCTCATCg                        >  1:990500/1‑40 (MQ=38)
                                                               |                                                          
CAGCCCCAGTACCTGCCATTCACGTTGCGTCAGCGGGCTGGTGCGGATCAGTTCAGGTACTTCAGGATGATTTAGCAGACGTTCAACGAAATTCTCATCGAAATGGGCGAATTTATGCCGAT  >  minE/2665474‑2665595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: