| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,665,765 | G→A | 29.8% | R749R (CGC→CGT) | malT ← | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,665,765 | 0 | G | A | 29.8% | 74.3 / 34.4 | 77 | R749R (CGC→CGT) | malT | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (13/41); new base A (14/9); total (27/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.58e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01 | |||||||||||
ACAAAATGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACG > minE/2665709‑2665827 | aCAAAATGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCa > 1:2119017/1‑69 (MQ=255)aCAAAATGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCa > 1:816973/1‑69 (MQ=255) aaaaTGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:1019679/68‑1 (MQ=255) aaaaTGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:2827823/68‑1 (MQ=255) aaaaTGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:2543565/68‑1 (MQ=255) aaaaTGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:2956349/68‑1 (MQ=255) aaaaTGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:311940/68‑1 (MQ=255) aaaaTGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:2094523/68‑1 (MQ=255) aaaaTGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:427473/68‑1 (MQ=255) aaaaTGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:1635224/68‑1 (MQ=255) aaaTGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:2500615/67‑1 (MQ=255) tGGCTGATAAATCCGTTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:3111702/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:2857803/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:1067115/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:2446617/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:2313283/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:3101974/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:1137371/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:769988/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:2061518/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:769137/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:1919456/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:537637/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:1043190/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:1565019/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:183450/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:721702/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:1797853/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:1894631/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:1669779/69‑1 (MQ=255) tGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTa < 1:629005/69‑1 (MQ=255) tCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCAc < 1:3236274/53‑1 (MQ=255) ccGGTACGATTCGCCAGTTTTAATGCGTCCAGCAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGc < 1:2512494/69‑1 (MQ=255) ccGGTACGATTCGCCAGTTTTAATGCGTCCAGCAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGc < 1:2722245/69‑1 (MQ=255) ccGGTACGATTCGCCAGTTTTAATGCGTCCAGCAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGc < 1:2327412/69‑1 (MQ=255) ccGGTACGATTCGCCAGTTTTAATGCGTCCAGCAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGc < 1:1672808/69‑1 (MQ=255) ccGGTACGATTCGCCAGTTTTAATGCGTCCAGCAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGc < 1:1819640/69‑1 (MQ=255) ccGGTACGATTCGCCAGTTTTAATGCGTCCAGCAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGc < 1:2025514/69‑1 (MQ=255) ccGGTACGATTCGCCAGTTTTAATGCGTCCAGCAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGc < 1:1835578/69‑1 (MQ=255) ccGGTACGATTCGCCAGTTTTAATGCGTCCAGCAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGc < 1:1918830/69‑1 (MQ=255) ggTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACt < 1:1555079/51‑1 (MQ=255) gTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAg < 1:1886446/69‑1 (MQ=255) gTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAg < 1:646196/69‑1 (MQ=255) gCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCa > 1:65042/1‑66 (MQ=255) gCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAg > 1:3055659/1‑67 (MQ=255) gCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAg > 1:1004594/1‑67 (MQ=255) gCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAg > 1:2952535/1‑67 (MQ=255) gCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAg > 1:997513/1‑67 (MQ=255) gCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAg > 1:2555425/1‑67 (MQ=255) gCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAg > 1:2741003/1‑67 (MQ=255) gCGATTCGCCAGTTTTAATGCGTCCAGCAACACGAGCTGGGCGTCACTTTTACGTCCGGCCTGCCAg > 1:2271405/1‑67 (MQ=255) tCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGtt > 1:241555/1‑69 (MQ=255) ttAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTAGGTCCGGCCTGCCAGTACAGTTGATTAaga > 1:619695/1‑67 (MQ=255) ttAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAagc > 1:1670587/1‑68 (MQ=255) cGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAAct < 1:1369852/69‑2 (MQ=255) cGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACa < 1:118314/69‑1 (MQ=255) cGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACa < 1:1381419/69‑1 (MQ=255) cGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACa < 1:3017649/69‑1 (MQ=255) cGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACa < 1:7528/69‑1 (MQ=255) cGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACa < 1:1403818/69‑1 (MQ=255) cGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACa < 1:446307/69‑1 (MQ=255) ccAGCAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGc < 1:1595500/41‑1 (MQ=255) cAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACa < 1:378119/65‑1 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:37811/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:1914964/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:3410885/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:2118903/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:2148214/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:1437868/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:673531/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:2210058/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:3048504/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:3008192/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:2453876/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:1264165/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:2912252/1‑69 (MQ=255) cAACACACGCTGGGCGTCACTTTTACGTCCGGCCTGACAGTACAGTTGATTAAGCAGCAACAGGTTACg > 1:1998945/1‑69 (MQ=255) | ACAAAATGGCTGATAAATCCGGTGCGATTCGCCAGTTTTAATGCGTCCAGCAACACGCGCTGGGCGTCACTTTTACGTCCGGCCTGCCAGTACAGTTGATTAAGCAGCAACAGGTTACG > minE/2665709‑2665827 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |