| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 227,569 | G→A | 24.4% | A121A (GCG→GCA) | crl → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 227,569 | 0 | G | A | 24.4% | 64.2 / 15.5 | 53 | A121A (GCG→GCA) | crl | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (32/8); new base A (2/11); total (34/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.25e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGA > minE/227506‑227623 | gCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGATTCTGAAGCTGGAACCGGCGgatga > 1:1552064/1‑69 (MQ=255)gCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGgatga > 1:1104842/1‑69 (MQ=255)gCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGgatga > 1:2785851/1‑69 (MQ=255)gCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGgatga > 1:2500118/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:2465730/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:716738/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:659103/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:2581773/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:2402411/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:1760950/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:1607577/1‑69 (MQ=255) tttCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt > 1:1587135/1‑69 (MQ=255) cACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAGCTGGAACCGGCAGATGATTTTCGTGa > 1:1628862/1‑68 (MQ=255) cACGAGAAGCTGCGTGAGCTGCTGGCGACGCTGAATCTGAAGCTGGAACCGGCAGATGATTTTCGTGa > 1:3347633/1‑68 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:2166785/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:141730/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:2646620/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:1467696/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:3123686/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:2056010/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:512026/1‑67 (MQ=255) cTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCgg > 1:164187/1‑67 (MQ=255) gCGTGAACTGCTGACGACGCTGAATGTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGaa > 1:2398965/1‑69 (MQ=255) gCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGaa > 1:1857766/1‑69 (MQ=255) tGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:401956/69‑1 (MQ=255) tGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:1470458/69‑1 (MQ=255) tGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:2828128/69‑1 (MQ=255) tGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGACGATTTTCGTGACGAGCCGGTGAAGtt < 1:1868453/69‑1 (MQ=255) gAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:2580942/68‑1 (MQ=255) ctgctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACg > 1:2427149/1‑69 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:347347/1‑68 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:985672/1‑68 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:2919848/1‑68 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:2331780/1‑68 (MQ=255) ctgACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc > 1:1691162/1‑68 (MQ=255) acgacgCTGAATCTGCAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt < 1:2023072/59‑1 (MQ=255) tCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTACCGGCGTGAGTGAAATGTGc > 1:1056970/1‑69 (MQ=255) tCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCg > 1:2054556/1‑55 (MQ=255) tCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGc > 1:2690388/1‑69 (MQ=255) tCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGc > 1:634754/1‑69 (MQ=255) tCTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:364027/69‑1 (MQ=38) tCTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:858964/69‑1 (MQ=38) tCTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:81105/69‑1 (MQ=38) tCTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:1258404/69‑1 (MQ=38) tCTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:1963546/69‑1 (MQ=38) tCTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:2751547/69‑1 (MQ=38) tCTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:3114617/69‑1 (MQ=38) tCTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:2999020/69‑1 (MQ=38) tCTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:2675119/69‑1 (MQ=38) cTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:542759/68‑1 (MQ=38) cTGAAGCTGGAACCGGCAGATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTGAATGAAATGTGc < 1:1173121/68‑1 (MQ=38) gCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGa < 1:3158986/67‑1 (MQ=255) gCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGa < 1:1656925/67‑1 (MQ=255) | GCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGA > minE/227506‑227623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |