| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,666,038 | A→C | 36.1% | I658M (ATT→ATG) | malT ← | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,666,038 | 0 | A | C | 36.1% | 21.1 / 26.6 | 36 | I658M (ATT→ATG) | malT | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Reads supporting (aligned to +/- strand): ref base A (7/16); new base C (5/8); total (12/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATGACGCAACCAGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGCCATTCCCCAGCAGGTTT > minE/2665986‑2666104 | tATGACGCAACCAGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTttgt < 1:98642/69‑1 (MQ=255)tATGACGCAACCAGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTttgt < 1:1278829/69‑1 (MQ=255) tGACGCAACCAGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTttgttg > 1:1012295/1‑69 (MQ=255) tGACGCAACCAGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTttgttg > 1:3389358/1‑69 (MQ=255) tGACGCAACCAGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTttgttg > 1:2563496/1‑69 (MQ=255) tGACGCAACCAGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTttgttg > 1:2373380/1‑69 (MQ=255) gCAACCAGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGc > 1:439293/1‑67 (MQ=255) aGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTACATCACCCTGACTTTGTTGGCg < 1:2528532/62‑1 (MQ=255) aGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCg < 1:990022/62‑1 (MQ=255) aGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCg < 1:943799/62‑1 (MQ=255) aGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCg < 1:714790/62‑1 (MQ=255) aGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCg < 1:1950325/62‑1 (MQ=255) aGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCg < 1:1235619/62‑1 (MQ=255) aGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCg < 1:1243512/62‑1 (MQ=255) aGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCg < 1:1542922/62‑1 (MQ=255) gcggcTTTATCGCCGGTCATTTGCCAGTAAATCACCCg > 1:800311/1‑38 (MQ=255) gcggcTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCt > 1:3388358/1‑68 (MQ=255) cggcTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCtgt < 1:104244/69‑1 (MQ=255) cggcTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCtgt < 1:2794671/69‑1 (MQ=255) cggcTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCtgt < 1:1266666/69‑1 (MQ=255) cTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGat < 1:1590991/69‑1 (MQ=255) cTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGat < 1:160144/69‑1 (MQ=255) cTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGat < 1:161392/69‑1 (MQ=255) cTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGat < 1:164245/69‑1 (MQ=255) cTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGat < 1:1029779/69‑1 (MQ=255) tttATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCg < 1:2465635/46‑1 (MQ=255) gCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGc < 1:1253995/68‑1 (MQ=255) gCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGc < 1:2797692/68‑1 (MQ=255) gCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGc < 1:1465368/68‑1 (MQ=255) ccGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCGtt > 1:2659436/1‑41 (MQ=255) ccGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGcc > 1:2246734/1‑68 (MQ=255) ccGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGCCa > 1:2994238/1‑69 (MQ=255) ccGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGCCa > 1:1734391/1‑69 (MQ=255) ccGGTCATTTGCCAGTACATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGCCa > 1:1517568/1‑69 (MQ=255) gTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGat < 1:1765773/58‑1 (MQ=255) aTTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGat < 1:2219801/55‑1 (MQ=255) aaaTCACCCGGACTTTGTTGGCGTTAGAGATCGAGTCGCTGTGATATTTGcc < 1:765997/52‑1 (MQ=255) aaaTCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGcc < 1:2302558/52‑1 (MQ=255) aaaTCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGcc < 1:1670208/52‑1 (MQ=255) aaaTCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGcc < 1:1199565/52‑1 (MQ=255) aaaTCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGcc < 1:642350/52‑1 (MQ=255) aaaTCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGcc < 1:2035092/52‑1 (MQ=255) aaaTCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGCCATTCCCCAGCAGGttt > 1:1011195/1‑68 (MQ=255) aaaTCACCCGGACTTTGTTGGCGTTAGAGATACAGTCg > 1:272068/1‑38 (MQ=255) aaaTCACCCGGACTTTGTTGGAGTTAGAGATCCAGTCGCTGTGATATTTGcc < 1:337057/52‑1 (MQ=255) aaTCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGcc < 1:2205501/51‑1 (MQ=255) | TATGACGCAACCAGTTGGCAGCGGCGGCTTTATCGCCGGTCATTTGCCAGTAAATCACCCGGACTTTGTTGGCGTTAGAGATCCAGTCGCTGTGATATTTGCCATTCCCCAGCAGGTTT > minE/2665986‑2666104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |