| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,668,916 | T→C | 46.4% | N98N (AAT→AAC) | malP → | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,668,916 | 0 | T | C | 46.4% | 11.1 / 95.4 | 110 | N98N (AAT→AAC) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base T (30/29); new base C (17/34); total (47/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CAACAACCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGG > minE/2668850‑2668980 | caacaaCCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCt > 1:2965096/1‑69 (MQ=255)caacaaCCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCt > 1:1705682/1‑69 (MQ=255)caacaaCCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCt > 1:442437/1‑69 (MQ=255)caacaaCCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCt > 1:1172741/1‑69 (MQ=255)caacaaCCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCt > 1:3431229/1‑69 (MQ=255)caacaaCCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCt > 1:2311255/1‑69 (MQ=255)caacaaCCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCt > 1:234748/1‑69 (MQ=255)caacaaCCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCt > 1:3180845/1‑69 (MQ=255) gAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACctgc > 1:1116569/1‑68 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2328443/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2436813/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2495481/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2089533/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:3016810/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:1721707/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:835965/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:744127/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:1198855/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1487049/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2295713/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2679668/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2657608/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1311655/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2651630/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1789402/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1830348/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:58653/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:601001/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCAGTTGgaaga < 1:1666557/66‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:3044352/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1070072/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1142514/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:2248760/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1033383/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:2704013/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1587224/69‑1 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:3059431/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:756681/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:1044882/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:2445708/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:3032252/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:82675/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:3123988/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:819042/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:2981368/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:1078616/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:759977/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:1617714/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:465814/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:532783/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:1886600/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:69342/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:178951/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:743560/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:1203123/51‑3 (MQ=255) ggTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:779705/51‑3 (MQ=255) gTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:3242227/50‑3 (MQ=255) gTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGActg < 1:1657725/50‑3 (MQ=255) aTCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATc < 1:783350/68‑1 (MQ=255) aTCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATc < 1:1083770/68‑1 (MQ=255) aTCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATc < 1:242886/68‑1 (MQ=255) tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:1021846/69‑1 (MQ=255) tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:2882794/69‑1 (MQ=255) tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:1009967/69‑1 (MQ=255) tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:2085300/69‑1 (MQ=255) cTTATGGCATCAATCTGACGGACCTGCTGgaagaa > 1:442157/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:893122/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1053360/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1067194/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1191208/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:142268/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1842175/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1915253/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1947373/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2052079/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2561809/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2971602/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:3010370/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2541689/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2435648/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:3075874/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2521904/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2510908/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:2984545/69‑1 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:1696829/69‑1 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTCgaagaa > 1:756918/1‑35 (MQ=255) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCt > 1:518066/1‑43 (MQ=37) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACgg > 1:3399072/1‑52 (MQ=255) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTgg > 1:394976/1‑60 (MQ=255) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2788214/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2774253/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:1327124/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2316240/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:775628/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:146719/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:1727355/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2396242/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2305251/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:3302335/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTg > 1:2032967/1‑68 (MQ=255) | CAACAACCTGTTGAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGG > minE/2668850‑2668980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |