| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,668,922 | G→T | 35.3% | T100T (ACG→ACT) | malP → | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,668,922 | 0 | G | T | 35.3% | 80.6 / 58.9 | 82 | T100T (ACG→ACT) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (24/29); new base T (17/12); total (41/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
GAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGC > minE/2668862‑2668984 | gAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACctgc > 1:1116569/1‑68 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:835965/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:3016810/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:1721707/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:1198855/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2495481/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2436813/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:744127/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2089533/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGgaag < 1:2328443/65‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2651630/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2657608/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2679668/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2750115/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1830348/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1789402/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:2295713/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:601001/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1311655/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:1487049/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCTGTTGgaaga < 1:58653/66‑1 (MQ=255) cTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAACCTGACTGACCAGTTGgaaga < 1:1666557/66‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1828709/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:3044352/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:2704013/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1488139/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1587224/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1142514/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1070072/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:2248760/69‑1 (MQ=255) tGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAagag < 1:1033383/69‑1 (MQ=255) aTCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATc < 1:1083770/68‑1 (MQ=255) aTCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATc < 1:783350/68‑1 (MQ=255) aTCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATc < 1:242886/68‑1 (MQ=255) aGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGAcc > 1:3033157/1‑69 (MQ=255) tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:2882794/69‑1 (MQ=255) tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:1021846/69‑1 (MQ=255) tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:1009967/69‑1 (MQ=255) tGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:2085300/69‑1 (MQ=255) aGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGgc < 1:749194/52‑1 (MQ=255) cTTATGGCATCAATCTGACGGACCTGCTGgaagaa > 1:442157/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2561809/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:3010370/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2971602/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:3075874/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1191208/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1067194/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1842175/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1915253/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1947373/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2052079/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2541689/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2521904/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2510908/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:2435648/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:1053360/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:893122/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGgaagaa > 1:142268/1‑35 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:1696829/69‑1 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:2440514/69‑1 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:2984545/69‑1 (MQ=255) cTTATGACATCAATCTGACGGACCTGCTCgaagaa > 1:756918/1‑35 (MQ=255) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCt > 1:518066/1‑43 (MQ=37) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACgg > 1:3399072/1‑52 (MQ=255) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTgg > 1:394976/1‑60 (MQ=255) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:1327124/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:775628/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2305251/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:908233/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:415492/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:3302335/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:146719/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:1727355/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2788214/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2774253/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2396242/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:228868/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2316240/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTg > 1:2032967/1‑68 (MQ=255) cTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCt > 1:2093861/1‑53 (MQ=255) cTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTggcggc > 1:2080996/1‑68 (MQ=255) cTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGACTGGGACGTCTgg > 1:268265/1‑64 (MQ=255) | GAATCTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGC > minE/2668862‑2668984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |