| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,668,958 | T→G | 51.5% | G112G (GGT→GGG) | malP → | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,668,958 | 0 | T | G | 51.5% | 10.0 / 71.3 | 66 | G112G (GGT→GGG) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base T (21/11); new base G (16/18); total (37/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTC > minE/2668903‑2669012 | cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:2984545/69‑1 (MQ=255)cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:2440514/69‑1 (MQ=255)cTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTgg < 1:1696829/69‑1 (MQ=255) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACgg > 1:3399072/1‑52 (MQ=255) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTgg > 1:394976/1‑60 (MQ=255) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2316240/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2788214/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:228868/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:1727355/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:3302335/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2305251/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2774253/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:2396242/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:146719/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:415492/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:775628/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:1327124/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTgg > 1:908233/1‑69 (MQ=38) tCAACCTGACTGACCTGTTGGAAGAAGAGATCGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTg > 1:2032967/1‑68 (MQ=255) cTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCt > 1:2093861/1‑53 (MQ=255) cTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTggcggc > 1:2080996/1‑68 (MQ=255) cTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGACTGGGACGTCTgg > 1:268265/1‑64 (MQ=255) ccTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCt > 1:3329031/1‑65 (MQ=255) ccTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCt > 1:2490389/1‑65 (MQ=255) ccTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCt > 1:1864458/1‑65 (MQ=255) ggAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTc > 1:3272042/1‑69 (MQ=255) ggAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTc > 1:2157809/1‑69 (MQ=255) agaagaGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAAt < 1:1578326/69‑1 (MQ=255) agaagaGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAAt < 1:802237/69‑1 (MQ=255) agaagaGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAAt < 1:1268625/69‑1 (MQ=255) gaagaGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTc > 1:2080344/1‑65 (MQ=255) gaagaGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTc < 1:3354730/65‑1 (MQ=255) gaagaGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTc > 1:3400941/1‑65 (MQ=255) aagaGATCGACCCGGCGCTGGGTAACGGTTGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTc < 1:1607216/64‑1 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:2943862/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:1125423/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:2717881/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:925839/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:2358094/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:1305457/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:1379072/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:1508591/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:1689459/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:1603952/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGt > 1:2564093/1‑49 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCa < 1:1010379/69‑1 (MQ=255) gagaTCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCa < 1:2113785/69‑1 (MQ=255) agaTCGACCCGGCGCTGGGTAACGGTGTTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCa < 1:348721/68‑1 (MQ=255) cGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGt < 1:1544793/69‑1 (MQ=255) cGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGt < 1:990753/69‑1 (MQ=255) cGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGt < 1:696264/69‑1 (MQ=255) cGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGt < 1:1379961/69‑1 (MQ=255) cGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGt < 1:374988/69‑1 (MQ=255) cGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGt < 1:1672163/69‑1 (MQ=255) cGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGt < 1:185356/69‑1 (MQ=255) cGACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGt < 1:2856922/69‑1 (MQ=255) cGACCCGGCGCTGGGGAACGGTGGGCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGt < 1:3149847/69‑1 (MQ=255) gACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCACTGGCAACTGTc < 1:1164305/69‑1 (MQ=255) gACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTc < 1:1724003/69‑1 (MQ=255) gACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTc < 1:3159480/69‑1 (MQ=255) gACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTc < 1:644968/69‑1 (MQ=255) gACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTc < 1:700714/69‑1 (MQ=255) gACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTc < 1:1971166/69‑1 (MQ=255) gACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTc < 1:215778/69‑1 (MQ=255) gACCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTc < 1:994335/69‑1 (MQ=255) aCCCGGCGCTGGGGAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTc < 1:3087095/68‑1 (MQ=255) | CTTATGACATCAATCTGACGGACCTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCGTGCTTCCTCGACTCAATGGCAACTGTC > minE/2668903‑2669012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |