| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,670,042 | C→A | 54.5% | Q474K (CAA→AAA) | malP → | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,670,042 | 0 | C | A | 54.5% | 3.3 / 86.0 | 77 | Q474K (CAA→AAA) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base C (27/8); new base A (36/6); total (63/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCCCACGTCGCTGGATCAAACAGTGCAACCCGGCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAAT > minE/2669979‑2670108 | aCCCCACGTCGCTGGATCAAACAGTGCAACCCGGCACTGGCGGCTCTGTTGGATAAATCACTGCaaa > 1:2384613/1‑67 (MQ=255)aCCCCACGTCGCTGGATCAAACAGTGCAACCCGGCACTGGCGGCTCTGTTGGATAAATCACTGCaaa > 1:182841/1‑67 (MQ=255)aCCCCACGTCGCTGGATCAAACAGTGCAACCCGGCACTGGCGGCTCTGTTGGATAAATCACTGCaaa > 1:1080327/1‑67 (MQ=255) gTGCAACCCGGCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCa < 1:969385/69‑1 (MQ=255) gTGCAACCCGGCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCa < 1:2405236/69‑1 (MQ=255) ggCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCaa < 1:3074922/69‑1 (MQ=255) ggCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCaa < 1:635487/69‑1 (MQ=255) cTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAAtat > 1:1052800/1‑66 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:1079934/63‑1 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:1721910/63‑1 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:2870701/63‑1 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:389496/63‑1 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:968692/63‑1 (MQ=255) ttgATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:2927205/60‑1 (MQ=255) aTAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgat < 1:2192440/68‑1 (MQ=255) aTAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgat < 1:2522555/68‑1 (MQ=255) aTAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgat < 1:2772844/68‑1 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgatgac > 1:688535/1‑68 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgatga > 1:1956939/1‑68 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgatga > 1:2691415/1‑68 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:1952942/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:1551003/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:3243569/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:2024135/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:950826/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:2323410/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:750010/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:2999630/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:2825944/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:112086/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCACGATCAGCTGAGCAATCTGGaaaa > 1:930625/1‑56 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1043337/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:355056/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2742749/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2784360/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:878009/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:849794/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:76264/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:3097427/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:3170136/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:463110/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:329216/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2018250/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1523850/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1472339/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1539425/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2733235/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1802426/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2234844/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:235889/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2365139/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:106832/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1014538/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1057164/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2435693/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2379719/1‑58 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:1914001/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:1195716/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:2537978/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:2965694/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:2605103/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:1291795/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:1402699/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:632710/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:633831/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:633875/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:2256384/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:1664573/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:1505732/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:634628/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:587923/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:481061/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:3426043/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:2792969/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:2662469/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:2666556/1‑69 (MQ=255) tGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAAt < 1:141997/69‑1 (MQ=255) | ACCCCACGTCGCTGGATCAAACAGTGCAACCCGGCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAAT > minE/2669979‑2670108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |