| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,670,071 | G→A | 43.2% | Q483Q (CAG→CAA) | malP → | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,670,071 | 0 | G | A | 43.2% | 32.4 / 71.4 | 97 | Q483Q (CAG→CAA) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (33/22); new base A (36/6); total (69/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.68e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGCAATATCGCGAGA > minE/2670011‑2670129 | ggCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCaa < 1:3074922/69‑1 (MQ=255)ggCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCaa < 1:635487/69‑1 (MQ=255) cTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAAtat > 1:1052800/1‑66 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:968692/63‑1 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:2870701/63‑1 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:1721910/63‑1 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:1079934/63‑1 (MQ=255) ttGGATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:389496/63‑1 (MQ=255) ttgATAAATCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGaaaaa < 1:2927205/60‑1 (MQ=255) aTAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgat < 1:2772844/68‑1 (MQ=255) aTAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgat < 1:2192440/68‑1 (MQ=255) aTAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgat < 1:2522555/68‑1 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgatgac > 1:688535/1‑68 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgatga > 1:1956939/1‑68 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCtgatga > 1:2691415/1‑68 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:750010/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:2323410/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:1551003/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:3243569/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:2024135/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:112086/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:2999630/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:1952942/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:950826/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTgatgat > 1:2825944/1‑69 (MQ=255) aaaTCACTGCAAAAAGAGTGGGCTAACGATCACGATCAGCTGAGCAATCTGGaaaa > 1:930625/1‑56 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2365139/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2733235/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1014538/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2379719/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2435693/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2742749/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2784360/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:3097427/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:3170136/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:329216/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:355056/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:463110/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:76264/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:849794/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:878009/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:235889/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1523850/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1539425/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1472339/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1802426/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2018250/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:106832/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1043337/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:2234844/1‑58 (MQ=255) aTCACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTc > 1:1057164/1‑58 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:2605103/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:632710/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:633831/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:633875/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:2965694/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:2537978/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:2256384/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:1402699/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:1914001/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:1291795/1‑69 (MQ=255) cACTGAAAAAAGAGTGGGCTAACGATCTCGATCAACTGATCAATCTGGAAAAATTCGCTGATGATGCGa > 1:1195716/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:2792969/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:1505732/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:634628/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:3426043/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:2666556/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:1664573/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:2662469/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:587923/1‑69 (MQ=255) aCTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGaa > 1:481061/1‑69 (MQ=255) tGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAAt < 1:141997/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:569463/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:1000716/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc > 1:1195831/1‑69 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:915280/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc > 1:2344881/1‑69 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:1879969/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc > 1:2277431/1‑69 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc > 1:2419905/1‑69 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc > 1:2467031/1‑69 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:2050577/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:2704532/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:270859/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:1989349/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc > 1:3372133/1‑69 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc > 1:2898478/1‑69 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc > 1:1690972/1‑69 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:3012221/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:1658553/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:327664/69‑1 (MQ=255) agTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:3287720/69‑1 (MQ=255) tGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGCaa < 1:1726621/69‑1 (MQ=255) tGGGCTAACGATCTCGATCAGCTGAGCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:156480/67‑1 (MQ=255) aTCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGCAATATCGCgaga < 1:1621636/69‑1 (MQ=255) ctcGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGCAATATCGCgaga > 1:71820/1‑67 (MQ=255) tCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGc < 1:2022957/50‑1 (MQ=255) | GGCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTCGATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGCAATATCGCGAGA > minE/2670011‑2670129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |