| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,671,973 | T→A | 21.2% | P316P (CCT→CCA) | malQ → | 4‑alpha‑glucanotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,671,973 | 0 | T | A | 21.2% | 214.7 / 50.6 | 118 | P316P (CCT→CCA) | malQ | 4‑alpha‑glucanotransferase |
| Reads supporting (aligned to +/- strand): ref base T (53/40); new base A (17/8); total (70/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
GGCAGCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGT > minE/2671910‑2672039 | ggcagCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATg < 1:1890032/69‑1 (MQ=255)ggcagCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATg < 1:573006/69‑1 (MQ=255) cagCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATg > 1:2165749/1‑67 (MQ=255) ccTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCag < 1:2941645/69‑1 (MQ=255) ccTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCag < 1:1609748/69‑1 (MQ=255) ccTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCag < 1:1755517/69‑1 (MQ=255) ccTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCag < 1:318394/69‑1 (MQ=255) cTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGc > 1:2313102/1‑65 (MQ=255) cTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGc > 1:2083171/1‑65 (MQ=255) cTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGc > 1:3267114/1‑65 (MQ=255) cTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGAATGGc > 1:2014824/1‑65 (MQ=255) cTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGCCTGGCCTGCATgg > 1:1244475/1‑64 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:286395/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:1982756/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:2935111/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:3261080/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:2015832/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:1807662/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:3239146/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:2254536/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:869916/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:2675275/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:3380222/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:1151071/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:127451/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:727943/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCagag > 1:567381/1‑69 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGTCTGGCCTGCATGGCCagag < 1:2265412/69‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGTCCagag < 1:5954/69‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCagag < 1:1634184/69‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCagag < 1:2120381/69‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCagag < 1:743438/69‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCagag < 1:1982792/69‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCagag < 1:725289/69‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCagag < 1:2951646/69‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCagag < 1:3214167/69‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCagag < 1:1686752/69‑1 (MQ=255) gATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCt > 1:1485939/1‑54 (MQ=255) gATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATg > 1:3177413/1‑69 (MQ=255) gATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATg > 1:1788612/1‑69 (MQ=255) gATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATg > 1:2002646/1‑69 (MQ=255) gATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATg > 1:2777258/1‑69 (MQ=255) aTGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATg < 1:564706/68‑1 (MQ=255) cTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAg < 1:1538843/69‑1 (MQ=255) cTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAg < 1:1083777/69‑1 (MQ=255) tGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACg > 1:2262945/1‑68 (MQ=255) tGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACg > 1:3214650/1‑68 (MQ=255) tGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACg > 1:2662840/1‑68 (MQ=255) tGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACg > 1:2715118/1‑68 (MQ=255) gCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTg < 1:3303846/69‑1 (MQ=255) gCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTg < 1:1450498/69‑1 (MQ=255) gCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTg < 1:829482/69‑1 (MQ=255) gCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTg < 1:914898/69‑1 (MQ=255) cAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCagag < 1:957442/51‑1 (MQ=255) aaGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTg > 1:187483/1‑36 (MQ=255) aaGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGAtt > 1:2401375/1‑66 (MQ=255) aaGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTc > 1:1267717/1‑67 (MQ=255) aaGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTc > 1:2339631/1‑67 (MQ=255) aaGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTc > 1:310243/1‑67 (MQ=255) aaGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTc > 1:1999061/1‑67 (MQ=255) gTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCa > 1:177596/1‑36 (MQ=255) gTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCAcc > 1:111679/1‑68 (MQ=255) gTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCAcc > 1:192830/1‑68 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATc > 1:149128/1‑49 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACg > 1:1055587/1‑55 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:2863515/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:1979843/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:871326/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:1279981/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:3156881/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:1955518/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:943149/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:2571517/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:2349858/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:110414/1‑69 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa > 1:524044/1‑68 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa > 1:3399989/1‑68 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa > 1:211141/1‑68 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa > 1:3060374/1‑68 (MQ=255) aTGTGCTGGGGCTGGCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGCCAGTTc < 1:3301534/69‑1 (MQ=255) aTGTGCTGGGGCTGGCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGCCAGTTc < 1:1418553/69‑1 (MQ=255) aTGTGCTGGGGCTGGCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGCCAGTTc < 1:1062787/69‑1 (MQ=255) tgtgcTGGGGCTGGCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGCCAGTTc < 1:2203210/65‑1 (MQ=255) tgtgcTGGGGCTGGCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGCCAGTTc < 1:2395338/65‑1 (MQ=255) tgtgcTGGGGCTGGCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGCCAGTTc < 1:1423309/65‑1 (MQ=255) ttgCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACAt < 1:1741562/67‑1 (MQ=255) tGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACAt < 1:175837/69‑1 (MQ=255) gCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATc > 1:1429702/1‑68 (MQ=255) gCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATc > 1:1581284/1‑68 (MQ=255) gCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATc > 1:2774419/1‑68 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGt < 1:1440449/68‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGt < 1:387511/68‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGt < 1:1363451/68‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGt < 1:1664577/68‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGt < 1:760776/68‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGt < 1:2372477/68‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGt < 1:19784/68‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGt < 1:198052/68‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGt < 1:23877/68‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTCCTGCGAAGAACATCGt < 1:3418466/68‑1 (MQ=255) | GGCAGCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGT > minE/2671910‑2672039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |