| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,674,145 | A→G | 25.5% | S131S (TCT→TCC) | gntT ← | gluconate transporter, high‑affinity GNT I system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,674,145 | 0 | A | G | 25.5% | 99.6 / 31.1 | 86 | S131S (TCT→TCC) | gntT | gluconate transporter, high‑affinity GNT I system |
| Reads supporting (aligned to +/- strand): ref base A (34/30); new base G (13/9); total (47/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
AAGCCGTGGGTCACAGACAGTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACAGGGCAAA > minE/2674080‑2674210 | aaGCCGTGGGTCACAGACAGTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGaa > 1:119893/1‑69 (MQ=255) acagacagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTa < 1:3057828/69‑1 (MQ=255) acagacagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTa < 1:2747646/69‑1 (MQ=255) acagacagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTa < 1:3067393/69‑1 (MQ=255) acagacagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTa < 1:1580859/69‑1 (MQ=255) acagacagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTa < 1:2609266/69‑1 (MQ=255) acagacagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTa < 1:1253674/69‑1 (MQ=255) cagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAacac < 1:611220/69‑1 (MQ=255) cagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAacac < 1:460079/69‑1 (MQ=255) cagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAacac < 1:1874922/69‑1 (MQ=255) cagTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAacac < 1:2637905/69‑1 (MQ=255) cagAGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAacac < 1:2282276/69‑1 (MQ=255) agTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGATTATTCGCAGaa > 1:3246604/1‑51 (MQ=255) agTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACAcc > 1:1645205/1‑69 (MQ=255) agTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACAcc > 1:2282726/1‑69 (MQ=255) gCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAg > 1:959646/1‑68 (MQ=255) gCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:1136927/1‑69 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCcgcg > 1:2831612/1‑53 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:2058612/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:2142432/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:2597656/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:2326988/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:2768318/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:3065220/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:181266/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:145989/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:3251202/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:3302393/1‑68 (MQ=255) cAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGc > 1:1104042/1‑68 (MQ=255) gccgccATTGGTACACCAACATACAGCAGTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCgg < 1:2958237/68‑1 (MQ=255) gccgccATTGGTACACCAACATACAGCAGTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCgg < 1:2962787/68‑1 (MQ=255) gccgccATTGGTACACCAACATACAGCAGTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCgg < 1:1905334/68‑1 (MQ=255) gccgccATTGGTACACCAACATACAGCAGTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCgg < 1:1580200/68‑1 (MQ=255) gccgccATTGGTACACCAACATACAGCAGTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCgg < 1:3146488/68‑1 (MQ=255) gccgccATTGGTACACCAACATACAGCAGTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCgg < 1:1169075/68‑1 (MQ=255) gccgccATTGGTACACCAACATACAGCAGTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCgg < 1:2283112/68‑1 (MQ=255) gccgccATTGGTACACCAACATACAGCAGTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCgg < 1:982687/68‑1 (MQ=255) ccgccATTGGTACACCAACATACAGCAGTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCgg < 1:2323989/67‑1 (MQ=255) gccATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGgcagc < 1:1078629/69‑1 (MQ=255) gccATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGgcagc < 1:318008/69‑1 (MQ=255) ccATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGgcagc < 1:1703837/68‑1 (MQ=255) ggTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAac > 1:1764698/1‑51 (MQ=255) ggTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAac > 1:469924/1‑51 (MQ=255) ggTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAac > 1:3404458/1‑51 (MQ=255) ggTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAac > 1:126009/1‑51 (MQ=255) ggTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGAGATGGTAAac > 1:1512578/1‑51 (MQ=255) cAACATAGAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGc < 1:1283357/69‑1 (MQ=255) cAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGc < 1:47417/69‑1 (MQ=255) cAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGc < 1:77555/69‑1 (MQ=255) cAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGc < 1:2813999/69‑1 (MQ=255) cAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGc < 1:1372846/69‑1 (MQ=255) cAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGc < 1:1063633/69‑1 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGcacga > 1:2456745/1‑55 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:1392446/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:3248395/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:1440676/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:3088840/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:583923/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:2364754/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:2928520/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:2883186/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:934138/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:2070587/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACtt > 1:2816770/1‑66 (MQ=255) gcagTGGAATATTCGCGGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACGAAACCCACt > 1:1868655/1‑65 (MQ=255) agTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg < 1:1657954/69‑1 (MQ=255) agTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg < 1:480525/69‑1 (MQ=255) agTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg < 1:2146106/69‑1 (MQ=255) agTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg < 1:3331743/69‑1 (MQ=255) agTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg < 1:939517/69‑1 (MQ=255) agTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg < 1:3268097/69‑1 (MQ=255) agTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg < 1:754393/69‑1 (MQ=255) agTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg < 1:892942/69‑1 (MQ=255) gTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCagag > 1:669231/1‑68 (MQ=255) gTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg > 1:25965/1‑68 (MQ=255) gTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAg > 1:300876/1‑68 (MQ=255) gAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACa > 1:2427420/1‑69 (MQ=255) gAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACa > 1:1835073/1‑69 (MQ=255) gAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACa > 1:2010490/1‑69 (MQ=255) gAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACa > 1:2241618/1‑69 (MQ=255) ttCGCAGAAGCCGCGATGGTAAACACCAGCGgcagc > 1:1450100/1‑36 (MQ=255) ttCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACAGGGc > 1:1269474/1‑68 (MQ=255) ttCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACAGGGc > 1:3130643/1‑68 (MQ=255) ttCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACAGGGc > 1:1786587/1‑68 (MQ=255) cGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACAGGGCaaa < 1:1419577/69‑1 (MQ=255) cGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACAGGGCaaa < 1:272200/69‑1 (MQ=255) | AAGCCGTGGGTCACAGACAGTGCAGCCGCCATTGGTACACCAACATACAGCAGTGGAATATTCGCAGAAGCCGCGATGGTAAACACCAGCGGCAGCATCAGCACAAAGCCCACTTCATAGAACAGGGCAAA > minE/2674080‑2674210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |