| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,676,470 | T→A | 83.4% | P73P (CCT→CCA) | bioH → | carboxylesterase of pimeloyl‑CoA synthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,676,470 | 0 | T | A | 83.4% | 83.1 / 9.4 | 42 | P73P (CCT→CCA) | bioH | carboxylesterase of pimeloyl‑CoA synthesis |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base A (15/20); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01 | |||||||||||
GGGATTTGGTGCGCTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGTT > minE/2676413‑2676529 | gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:2797456/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:944463/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:756307/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:646395/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:494817/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:380540/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:365773/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:218651/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:2038306/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:12236/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:1513076/1‑68 (MQ=255)gggATTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCa > 1:206680/1‑68 (MQ=255) aTTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCATTTg > 1:2092178/1‑69 (MQ=255) aTTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCATTTg > 1:2949905/1‑69 (MQ=255) aTTTGGTGCGCTATCACTTGCTGATATGGCCGAAGTCGTGCTGCGACAGGCACCAGATAAAGCCATTTg > 1:248100/1‑69 (MQ=255) gcgcTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGc < 1:3217080/69‑1 (MQ=255) gcgcTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGc < 1:808959/69‑1 (MQ=255) gcgcTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGc < 1:2145700/69‑1 (MQ=255) gcgcTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGc < 1:2362119/69‑1 (MQ=255) gCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCtggt > 1:1237504/1‑68 (MQ=255) gCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCtggt > 1:627401/1‑68 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGTAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:748157/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2200427/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:1162395/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:860025/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:840830/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2193695/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:700041/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:559603/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2432183/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:183190/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:322476/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2406677/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2879801/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:1045963/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2640189/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2614997/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:2600790/69‑1 (MQ=255) aCAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCCGGCTGGTGGCAAGCCAGATTGCGtt < 1:3068496/69‑1 (MQ=255) cAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCATATTGCGtt < 1:1721778/68‑1 (MQ=255) cAGGCACCAGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGtt < 1:836879/68‑1 (MQ=255) aGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAg < 1:669730/59‑1 (MQ=255) | GGGATTTGGTGCGCTGTCACTTGCTGATATGGCCGAAGCCGTGCTGCAACAGGCACCTGATAAAGCCATTTGGTTAGGCTGGAGTCTGGGCGGGCTGGTGGCAAGCCAGATTGCGTT > minE/2676413‑2676529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |